BMRB Entry 26969

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26969

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone resonance assignments of the Pseudomonas aeruginosa major pilin PilA from strain PA14
Deposition date:
2016-12-07
Original release date:
2018-06-26
Authors:
Nguyen, Ylan; McNicholl, Tyler; Akimoto, Madoka; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori
Citation:

Citation: Nguyen, Ylan; Boulton, Stephen; McNicholl, E Tyler; Akimoto, Madoka; Harvey, Hanjeong; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori. "A Highly Dynamic Loop of the Pseudomonas aeruginosa PA14 Type IV Pilin Is Essential for Pilus Assembly."  ACS Infect. Dis. 4, 936-943 (2018).
PubMed: 29342354

Assembly members:

Assembly members:
PilA, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PilA: GIDPFTIRARVTEGVGLAAS AKTLIGDSSATAGELAASAR VWNAQAGNAGATSKYVTSVR IAEATGEITVTFNATNVGNI PADSTLVFTPYVQNAAGAPT QLGASYAAGVTGSIDWGCAS ESNAVSSGPDRNMPALTAGT LPARFAPSECR

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PilA1

Entities:

Entity 1, PilA 151 residues - Formula weight is not available

residues 1-6 are artifacts of affinity tag cleavage

1   GLYILEASPPROPHETHRILEARGALAARG
2   VALTHRGLUGLYVALGLYLEUALAALASER
3   ALALYSTHRLEUILEGLYASPSERSERALA
4   THRALAGLYGLULEUALAALASERALAARG
5   VALTRPASNALAGLNALAGLYASNALAGLY
6   ALATHRSERLYSTYRVALTHRSERVALARG
7   ILEALAGLUALATHRGLYGLUILETHRVAL
8   THRPHEASNALATHRASNVALGLYASNILE
9   PROALAASPSERTHRLEUVALPHETHRPRO
10   TYRVALGLNASNALAALAGLYALAPROTHR
11   GLNLEUGLYALASERTYRALAALAGLYVAL
12   THRGLYSERILEASPTRPGLYCYSALASER
13   GLUSERASNALAVALSERSERGLYPROASP
14   ARGASNMETPROALALEUTHRALAGLYTHR
15   LEUPROALAARGPHEALAPROSERGLUCYS
16   ARG

Related Database Links:

UNP A0A0H2ZFW8
AlphaFold A0A0H2ZFW8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks