BMRB Entry 26932

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26932

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for MTTTm
Deposition date:
2016-11-03
Original release date:
2017-03-27
Authors:
Burban, David; Jennings, Patricia
Citation:

Citation: Burban, David; Jennings, Patricia. "Backbone assignments for the SPOUT methyltransferase MTT Tm , a knotted protein from Thermotoga maritima"  Biomol. NMR Assign. 11, 151-154 (2017).
PubMed: 28284017

Assembly members:

Assembly members:
MTTTm, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MTTTm: SLRVRIAVIGKLDGFIKEGI KHYEKFLRRFCKPEVLEIKR VHRGSIEEIVRKETEDLTNR ILPGSFVMVMDKRGEEVSSE EFADFLKDLEMKGKDITILI GGPYGLNEEIFAKAHRVFSL SKMTFTHGMTVLIVLEQIFR AFKIIHGENYHYEGGSHHHH HH

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MTTTm monomer1

Entities:

Entity 1, MTTTm monomer 162 residues - Formula weight is not available

1   SERLEUARGVALARGILEALAVALILEGLY
2   LYSLEUASPGLYPHEILELYSGLUGLYILE
3   LYSHISTYRGLULYSPHELEUARGARGPHE
4   CYSLYSPROGLUVALLEUGLUILELYSARG
5   VALHISARGGLYSERILEGLUGLUILEVAL
6   ARGLYSGLUTHRGLUASPLEUTHRASNARG
7   ILELEUPROGLYSERPHEVALMETVALMET
8   ASPLYSARGGLYGLUGLUVALSERSERGLU
9   GLUPHEALAASPPHELEULYSASPLEUGLU
10   METLYSGLYLYSASPILETHRILELEUILE
11   GLYGLYPROTYRGLYLEUASNGLUGLUILE
12   PHEALALYSALAHISARGVALPHESERLEU
13   SERLYSMETTHRPHETHRHISGLYMETTHR
14   VALLEUILEVALLEUGLUGLNILEPHEARG
15   ALAPHELYSILEILEHISGLYGLUASNTYR
16   HISTYRGLUGLYGLYSERHISHISHISHIS
17   HISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks