BMRB Entry 26919

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PDB ID: 5o2y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26919
MolProbity Validation Chart

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Title:
1H, 15N and 13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca Type 2 Secretion System
Deposition date:
2016-10-19
Original release date:
2017-04-26
Authors:
Lopez-Castilla, Aracelys; Vitorge, Bruno; Khoury, Lea; Morellet, Nelly; Francetic, Olivera; Izadi-Pruneyre, Nadia
Citation:

Citation: Lopez-Castilla, Aracelys; Vitorge, Bruno; Khoury, Lea; Morellet, Nelly; Francetic, Olivera; Izadi-Pruneyre, Nadia. "1H, 15N and 13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca type 2 secretion system"  Biomol. NMR Assign. 11, 155-158 (2017).
PubMed: 28258547

Assembly members:

Assembly members:
PulG, polymer, 116 residues, 12912.1838 Da.

Natural source:

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 571   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella oxytoca

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCHAP7013

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PulG: MGNKEKADRQKVVSDLVALE GALDMYKLDNSRYPTTEQGL QALVSAPSAEPHARNYPEGG YIRRLPQDPWGSDYQLLSPG QHGQVDIFSLGPDGVPESND DIGNWTIGFHHHHHHK

Data sets:
Data typeCount
13C chemical shifts421
15N chemical shifts103
1H chemical shifts630

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PulG1

Entities:

Entity 1, PulG 116 residues - 12912.1838 Da.

1   METGLYASNLYSGLULYSALAASPARGGLN
2   LYSVALVALSERASPLEUVALALALEUGLU
3   GLYALALEUASPMETTYRLYSLEUASPASN
4   SERARGTYRPROTHRTHRGLUGLNGLYLEU
5   GLNALALEUVALSERALAPROSERALAGLU
6   PROHISALAARGASNTYRPROGLUGLYGLY
7   TYRILEARGARGLEUPROGLNASPPROTRP
8   GLYSERASPTYRGLNLEULEUSERPROGLY
9   GLNHISGLYGLNVALASPILEPHESERLEU
10   GLYPROASPGLYVALPROGLUSERASNASP
11   ASPILEGLYASNTRPTHRILEGLYPHEHIS
12   HISHISHISHISHISLYS

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