BMRB Entry 26916

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26916

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Title:
Recombinant Human Granulocyte-Colony Stimulating Factor variant C3 (G-CSFC3) backbone assignments
Deposition date:
2016-10-08
Original release date:
2016-10-28
Authors:
Thomas, Rhys
Citation:

Citation: Thomas, Rhys; Kalverda, Arnout; Stewart, Katie; Buchanan, Andrew; Radford, Sheena; Brockwell, David. "Elucidating a role for protein dynamics in the aggregation of a Granulocyte-Colony Stimulating Factor variant"  .

Assembly members:

Assembly members:
G-CSFC3, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G-CSFC3: MTPLGPASSLPQSFLLKGLE QVRKIQGDGAALQEKLCATY KLCHPEELVLLGHSLGIPRA PLSSCPSQALRLAGCLSQLH SGLLLYQGLLQALEGISPEL GPTLDTLQLDVADFATTIWQ QMEELGMAPALQPTQGAMPA FASAFQRRAGGVLVASHLQS FLEVSYRVLRHLAQP

Data typeCount
13C chemical shifts909
15N chemical shifts324
1H chemical shifts324

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1G-CSFC31

Entities:

Entity 1, G-CSFC3 175 residues - Formula weight is not available

G-CSFC3 is identical to wild type human G-CSF (expressed recombinantly in E. coli) apart from substitutions C18G, W59R, Q71R and F84L.

1   METTHRPROLEUGLYPROALASERSERLEU
2   PROGLNSERPHELEULEULYSGLYLEUGLU
3   GLNVALARGLYSILEGLNGLYASPGLYALA
4   ALALEUGLNGLULYSLEUCYSALATHRTYR
5   LYSLEUCYSHISPROGLUGLULEUVALLEU
6   LEUGLYHISSERLEUGLYILEPROARGALA
7   PROLEUSERSERCYSPROSERGLNALALEU
8   ARGLEUALAGLYCYSLEUSERGLNLEUHIS
9   SERGLYLEULEULEUTYRGLNGLYLEULEU
10   GLNALALEUGLUGLYILESERPROGLULEU
11   GLYPROTHRLEUASPTHRLEUGLNLEUASP
12   VALALAASPPHEALATHRTHRILETRPGLN
13   GLNMETGLUGLULEUGLYMETALAPROALA
14   LEUGLNPROTHRGLNGLYALAMETPROALA
15   PHEALASERALAPHEGLNARGARGALAGLY
16   GLYVALLEUVALALASERHISLEUGLNSER
17   PHELEUGLUVALSERTYRARGVALLEUARG
18   HISLEUALAGLNPRO

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks