BMRB Entry 26909

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26909

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Title:
N-terminal domain of the E. coli DNA polymerase III delta subunit
Deposition date:
2016-09-26
Original release date:
2017-05-24
Authors:
Alyami, Esmael; Rizzo, Alessandro; Korzhnev, Dmitry
Citation:

Citation: Alyami, Esmael; Rizzo, Alessandro; Beuning, Penny; Korzhnev, Dmitry. "NMR resonance assignments for the N-terminal domain of the delta subunit of the E. coli gamma clamp loader complex"  Biomol. NMR Assignments 11, 169-173 (2017).
PubMed: 28265855

Assembly members:

Assembly members:
E_coli_DNA_polymerase_III_delta_subunit_(residues_1-140), polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E_coli_DNA_polymerase_III_delta_subunit_(residues_1-140): GSHMIRLYPEQLRAQLNEGL RAAYLLLGNDPLLLQESQDA VRQVAAAQGFEEHHTFSIDP NTDWNAIFSLCQAMSLFASR QTLLLLLPENGPNAAINEQL LTLTGLLHDDLLLIVRGNKL SKAQENAAWFTALANRSVQV TCQ

Data sets:
Data typeCount
13C chemical shifts587
15N chemical shifts148
1H chemical shifts953

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1E coli DNA polymerase III delta subunit (residues 1-140)1

Entities:

Entity 1, E coli DNA polymerase III delta subunit (residues 1-140) 143 residues - Formula weight is not available

GSH are cloning artifacts. The native residues are M1-Q140

1   GLYSERHISMETILEARGLEUTYRPROGLU
2   GLNLEUARGALAGLNLEUASNGLUGLYLEU
3   ARGALAALATYRLEULEULEUGLYASNASP
4   PROLEULEULEUGLNGLUSERGLNASPALA
5   VALARGGLNVALALAALAALAGLNGLYPHE
6   GLUGLUHISHISTHRPHESERILEASPPRO
7   ASNTHRASPTRPASNALAILEPHESERLEU
8   CYSGLNALAMETSERLEUPHEALASERARG
9   GLNTHRLEULEULEULEULEUPROGLUASN
10   GLYPROASNALAALAILEASNGLUGLNLEU
11   LEUTHRLEUTHRGLYLEULEUHISASPASP
12   LEULEULEUILEVALARGGLYASNLYSLEU
13   SERLYSALAGLNGLUASNALAALATRPPHE
14   THRALALEUALAASNARGSERVALGLNVAL
15   THRCYSGLN

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