BMRB Entry 26908

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26908

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Title:
Backbone 1H, 13C, 15N chemical shift assignments of portions of Thermotoga maritima flagellar motor proteins FliG (N-terminal domain; FliGN) and FliF (C-terminal domain; FliFC) in complex
Deposition date:
2016-09-23
Original release date:
2017-02-15
Authors:
Levenson, Robert; Dahlquist, Frederick
Citation:

Citation: Lynch, Michael; Levenson, Robert; Kim, Eun; Sircar, Ria; Blair, David; Dahlquist, Frederick; Crane, Brian. "Co-Folding of a FliF-FliG Split Domain Forms the Basis of the MS:C Ring Interface within the Bacterial Flagellar Motor"  Structure 25, 317-328 (2017).
PubMed: 28089452

Assembly members:

Assembly members:
FliGn, polymer, 104 residues, Formula weight is not available
FliFc, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJY5

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FliGn: GHMPEKKIDGRRKAAVLLVA LGPEKAAQVMKHLDEETVEQ LVVEIANIGRVTPEEKKQVL EEFLSLAKAKEMISEGGIEY AKKVLEKAFGPERARKIIER LTSS
FliFc: MVSPEEKELLELLEELENIF SRSPSDIAEIVRLWFFERGL EDHHHHHHHHASENLYFQ

Data sets:
Data typeCount
13C chemical shifts267
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FliGn1
2FliFc2

Entities:

Entity 1, FliGn 104 residues - Formula weight is not available

1   GLYHISMETPROGLULYSLYSILEASPGLY
2   ARGARGLYSALAALAVALLEULEUVALALA
3   LEUGLYPROGLULYSALAALAGLNVALMET
4   LYSHISLEUASPGLUGLUTHRVALGLUGLN
5   LEUVALVALGLUILEALAASNILEGLYARG
6   VALTHRPROGLUGLULYSLYSGLNVALLEU
7   GLUGLUPHELEUSERLEUALALYSALALYS
8   GLUMETILESERGLUGLYGLYILEGLUTYR
9   ALALYSLYSVALLEUGLULYSALAPHEGLY
10   PROGLUARGALAARGLYSILEILEGLUARG
11   LEUTHRSERSER

Entity 2, FliFc 58 residues - Formula weight is not available

1   METVALSERPROGLUGLULYSGLULEULEU
2   GLULEULEUGLUGLULEUGLUASNILEPHE
3   SERARGSERPROSERASPILEALAGLUILE
4   VALARGLEUTRPPHEPHEGLUARGGLYLEU
5   GLUASPHISHISHISHISHISHISHISHIS
6   ALASERGLUASNLEUTYRPHEGLN

Related Database Links:

BMRB 18309
GB AAD35312 ABQ46724 ACB09081 ADA66942 AGL49144 AAD35313 ABQ46723 ACB09080 ADA66943 AGL49145
REF NP_228035 WP_004082903 WP_008193872 WP_011943308 WP_012310706 NP_228036 WP_004082905 WP_008193870 WP_011943307 WP_012310705
SP Q9WY63
AlphaFold Q9WY63

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks