BMRB Entry 26876

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26876

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone chemical shift assignments of N14D mutant of Vibrio cholerae peptidyl-tRNA hydrolase
Deposition date:
2016-08-15
Original release date:
2017-02-16
Authors:
Kabra, Ashish; Shahid, Salman; Arora, Ashish
Citation:

Citation: Kabra, Ashish; Shahid, Salman; Pal, Ravikant; Yadav, Rahul; Pulavarti, SVSRK; Jain, Anupam; Tripathi, Sarita; Arora, Ashish. "Unraveling the stereochemical and dynamic aspects of the catalytic site of bacterial peptidyl-tRNA hydrolase"  RNA 23, 202-216 (2017).
PubMed: 28096445

Assembly members:

Assembly members:
VcPth-N14D_mutant, polymer, 199 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-NH6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VcPth-N14D_mutant: GAMVSQPIKLLVGLADPGPE YAKTRHNAGAWVVEELARIH NVTLKNEPKFFGLTGRLLIN SQELRVLIPTTFMNLSGKAI AALANFYQIKPEEIMVAHDE LDLPPGVAKFKQGGGHGGHN GLKDTISKLGNNKEFYRLRL GIGHPGHKDKVAGYVLGKAP AKEQECLDAAVDESVRCLEI LMKDGLTKAQNRLHTFKAE

Data sets:
Data typeCount
13C chemical shifts552
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VcPth-N14D mutant1

Entities:

Entity 1, VcPth-N14D mutant 199 residues - Formula weight is not available

1   GLYALAMETVALSERGLNPROILELYSLEU
2   LEUVALGLYLEUALAASPPROGLYPROGLU
3   TYRALALYSTHRARGHISASNALAGLYALA
4   TRPVALVALGLUGLULEUALAARGILEHIS
5   ASNVALTHRLEULYSASNGLUPROLYSPHE
6   PHEGLYLEUTHRGLYARGLEULEUILEASN
7   SERGLNGLULEUARGVALLEUILEPROTHR
8   THRPHEMETASNLEUSERGLYLYSALAILE
9   ALAALALEUALAASNPHETYRGLNILELYS
10   PROGLUGLUILEMETVALALAHISASPGLU
11   LEUASPLEUPROPROGLYVALALALYSPHE
12   LYSGLNGLYGLYGLYHISGLYGLYHISASN
13   GLYLEULYSASPTHRILESERLYSLEUGLY
14   ASNASNLYSGLUPHETYRARGLEUARGLEU
15   GLYILEGLYHISPROGLYHISLYSASPLYS
16   VALALAGLYTYRVALLEUGLYLYSALAPRO
17   ALALYSGLUGLNGLUCYSLEUASPALAALA
18   VALASPGLUSERVALARGCYSLEUGLUILE
19   LEUMETLYSASPGLYLEUTHRLYSALAGLN
20   ASNARGLEUHISTHRPHELYSALAGLU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks