BMRB Entry 26822

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26822

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of Human integrin alpha1 I domain mutant E317A
Deposition date:
2016-06-20
Original release date:
2016-08-31
Authors:
Nunes, Ana Monica; Zhu, Jie; Jezioro, Jacqueline; Minetti, Conceicao; Remeta, David; Farndale, Richard; Hamaia, Samir; Baum, Jean
Citation:

Citation: Nunes, Ana Monica; Zhu, Jie; Jezioro, Jacqueline; Minetti, Conceicao; Remeta, David; Farndale, Richard; Hamaia, Samir; Baum, Jean. "Intrinsic local destabilization of the C-terminus predisposes integrin alpha1 I domain to a conformational switch induced by collagen binding"  Protein Sci. 25, 1672-1681 (2016).
PubMed: 27342747

Assembly members:

Assembly members:
integrin_alpha1_I_domain_mutant_E317A, polymer, 216 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_Dest_42

Entity Sequences (FASTA):

Entity Sequences (FASTA):
integrin_alpha1_I_domain_mutant_E317A: MNHHHHHHHHHHTSLYKKAG FTQLDIVIVLDGSNSIYPWD SVTAFLNDLLERMDIGPKQT QVGIVQYGENVTHEFNLNKY SSTEEVLVAAKKIVQRGGRQ TMTALGIDTARKEAFTEARG ARRGVKKVMVIVTDGESHDN HRLKKVIQDCEDENIQRFSI AILGSYNRGNLSTEKFVEEI KSIASEPTEKHFFNVSDALA LVTIVKTLGERIFALE

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts166
1H chemical shifts166

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1a1I_E317A1

Entities:

Entity 1, a1I_E317A 216 residues - Formula weight is not available

1   METASNHISHISHISHISHISHISHISHIS
2   HISHISTHRSERLEUTYRLYSLYSALAGLY
3   PHETHRGLNLEUASPILEVALILEVALLEU
4   ASPGLYSERASNSERILETYRPROTRPASP
5   SERVALTHRALAPHELEUASNASPLEULEU
6   GLUARGMETASPILEGLYPROLYSGLNTHR
7   GLNVALGLYILEVALGLNTYRGLYGLUASN
8   VALTHRHISGLUPHEASNLEUASNLYSTYR
9   SERSERTHRGLUGLUVALLEUVALALAALA
10   LYSLYSILEVALGLNARGGLYGLYARGGLN
11   THRMETTHRALALEUGLYILEASPTHRALA
12   ARGLYSGLUALAPHETHRGLUALAARGGLY
13   ALAARGARGGLYVALLYSLYSVALMETVAL
14   ILEVALTHRASPGLYGLUSERHISASPASN
15   HISARGLEULYSLYSVALILEGLNASPCYS
16   GLUASPGLUASNILEGLNARGPHESERILE
17   ALAILELEUGLYSERTYRASNARGGLYASN
18   LEUSERTHRGLULYSPHEVALGLUGLUILE
19   LYSSERILEALASERGLUPROTHRGLULYS
20   HISPHEPHEASNVALSERASPALALEUALA
21   LEUVALTHRILEVALLYSTHRLEUGLYGLU
22   ARGILEPHEALALEUGLU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks