BMRB Entry 26787

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26787

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human RIT1
Deposition date:
2016-04-25
Original release date:
2016-09-02
Authors:
Fang, Zhenhao; Marshall, Christopher; Yin, Jiani; Mazhab-Jafari, Mohammad; Gasmi-Seabrook, Genevieve; Smith, Matthew; Nishikawa, Tadateru; Xu, Yang; Neel, Benjamin; Ikura, Mitsuhiko
Citation:

Citation: Fang, Zhenhao; Marshall, Christopher; Yin, Jiani; Mazhab-Jafari, Mohammad; Gasmi-Seabrook, Genevieve; Smith, Matthew; Xu, Yang; Neel, Benjamin; Ikura, Mitsuhiko. "Biochemical Classification of Disease-associated Mutants of RAS-like Protein Expressed in Many Tissues (RIT1)"  J. Biol. Chem. 291, 15641-15652 (2016).
PubMed: 27226556

Assembly members:

Assembly members:
RIT1, polymer, 179 residues, 20705.59 Da.
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RIT1: GSHMGLSREYKLVMLGAGGV GKSAMTMQFISHRFPEDHDP TIEDAYKIRIRIDDEPANLD ILDTAGQAEFTAMRDQYMRA GEGFIICYSITDRRSFHEVR EFKQLIYRVRRTDDTPVVLV GNKSDLKQLRQVTKEEGLAL AREFSCPFFETSAAYRYYID DVFHALVREIRRKEKEAVL

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts128
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RIT11
2GDP2
3MG3

Entities:

Entity 1, RIT1 179 residues - 20705.59 Da.

Residues 13-16 represent a vector derived sequence. Residues 17-191 is RIT1 sequence that contains G domain.

1   GLYSERHISMETGLYLEUSERARGGLUTYR
2   LYSLEUVALMETLEUGLYALAGLYGLYVAL
3   GLYLYSSERALAMETTHRMETGLNPHEILE
4   SERHISARGPHEPROGLUASPHISASPPRO
5   THRILEGLUASPALATYRLYSILEARGILE
6   ARGILEASPASPGLUPROALAASNLEUASP
7   ILELEUASPTHRALAGLYGLNALAGLUPHE
8   THRALAMETARGASPGLNTYRMETARGALA
9   GLYGLUGLYPHEILEILECYSTYRSERILE
10   THRASPARGARGSERPHEHISGLUVALARG
11   GLUPHELYSGLNLEUILETYRARGVALARG
12   ARGTHRASPASPTHRPROVALVALLEUVAL
13   GLYASNLYSSERASPLEULYSGLNLEUARG
14   GLNVALTHRLYSGLUGLUGLYLEUALALEU
15   ALAARGGLUPHESERCYSPROPHEPHEGLU
16   THRSERALAALATYRARGTYRTYRILEASP
17   ASPVALPHEHISALALEUVALARGGLUILE
18   ARGARGLYSGLULYSGLUALAVALLEU

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, MG - Mg - 24.305 Da.

1   MG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks