BMRB Entry 26765

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26765

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Title:
NMR backbone 1H, 13C and 15N chemical shift assignments of human S-phase kinase-associated protein 1 (Skp1)
Deposition date:
2016-03-22
Original release date:
2017-03-02
Authors:
Kachariya, Nitin Nathubhai; Kumar, Ashutosh
Citation:

Citation: Kachariya, Nitin; Dantu, Sarath; Kumar, Ashutosh. "Backbone and side chain assignments of human cell cycle regulatory protein S-phase kinase-associated protein 1"  J. Biomol. NMR 10, 351-355 (2016).
PubMed: 27394725

Assembly members:

Assembly members:
Skp1, polymer, 168 residues, 19289 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Skp1: GPLGSMPSIKLQSSDGEIFE VDVEIAKQSVTIKTMLEDLG MDDEGDDDPVPLPNVNAAIL KKVIQWCTHHKDDPPPPEDD ENKEKRTDDIPVWDQEFLKV DQGTLFELILAANYLDIKGL LDVTCKTVANMIKGKTPEEI RKTFNIKNDFTEEEEAQVRK ENQWCEEK

Data sets:
Data typeCount
13C chemical shifts607
15N chemical shifts149
1H chemical shifts964

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Skp11

Entities:

Entity 1, Skp1 168 residues - 19289 Da.

"GPLGS" five extra residues are present at N terminal of Skp1

1   GLYPROLEUGLYSERMETPROSERILELYS
2   LEUGLNSERSERASPGLYGLUILEPHEGLU
3   VALASPVALGLUILEALALYSGLNSERVAL
4   THRILELYSTHRMETLEUGLUASPLEUGLY
5   METASPASPGLUGLYASPASPASPPROVAL
6   PROLEUPROASNVALASNALAALAILELEU
7   LYSLYSVALILEGLNTRPCYSTHRHISHIS
8   LYSASPASPPROPROPROPROGLUASPASP
9   GLUASNLYSGLULYSARGTHRASPASPILE
10   PROVALTRPASPGLNGLUPHELEULYSVAL
11   ASPGLNGLYTHRLEUPHEGLULEUILELEU
12   ALAALAASNTYRLEUASPILELYSGLYLEU
13   LEUASPVALTHRCYSLYSTHRVALALAASN
14   METILELYSGLYLYSTHRPROGLUGLUILE
15   ARGLYSTHRPHEASNILELYSASNASPPHE
16   THRGLUGLUGLUGLUALAGLNVALARGLYS
17   GLUASNGLNTRPCYSGLUGLULYS

Related Database Links:

NCBI NP_733779.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks