BMRB Entry 26745

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26745

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Title:
Backbone 1H, 13C, and 15N assignments for Sortase C1 (SrtC1) from Streptococcus pneumoniae
Deposition date:
2016-02-13
Original release date:
2016-07-14
Authors:
Jacobitz, Alex; Clubb, Robert
Citation:

Citation: Jacobitz, Alex; Naziga, Emmanuel; Sung Wook, Yi; McConnell, Scott; Peterson, Robert; Jung, Michael; Clubb, Robert; Wereszczynski, Jeff. "The "Lid" in the Streptococcus pneumoniae SrtC1 Sortase Adopts a Rigid Structure that Regulates Substrate Access to the Active Site"  J. Phys. Chem. B 120, 8302-8312 (2016).
PubMed: 27109553

Assembly members:

Assembly members:
SrtC1, polymer, 213 residues, 23812.9 Da.

Natural source:

Natural source:   Common Name: fermicutes   Taxonomy ID: 170187   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pESUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SrtC1: SESNQQIADFDKEKATLDEA DIDERMKLAQAFNDSLNNVV SGDPWSEEMKKKGRAEYARM LEIHERMGHVEIPVIDVDLP VYAGTAEEVLQQGAGHLEGT SLPIGGNSTHAVITAHTGLP TAKMFTDLTKLKVGDKFYVH NIKEVMAYQVDQVKVIEPTN FDDLLIVPGHDYVTLLTCTP YMINTHRLLVRGHRIPYVAE VEEEFIAANKLSH

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts192
1H chemical shifts339

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SrtC11

Entities:

Entity 1, SrtC1 213 residues - 23812.9 Da.

Sequence matches PDB ID 2W1J. First 15 residues in this sequence are part of a membrane anchor and were removed to generate a soluble construct. First residue, S-16, is a remnant of a cleaved purification tag.

1   SERGLUSERASNGLNGLNILEALAASPPHE
2   ASPLYSGLULYSALATHRLEUASPGLUALA
3   ASPILEASPGLUARGMETLYSLEUALAGLN
4   ALAPHEASNASPSERLEUASNASNVALVAL
5   SERGLYASPPROTRPSERGLUGLUMETLYS
6   LYSLYSGLYARGALAGLUTYRALAARGMET
7   LEUGLUILEHISGLUARGMETGLYHISVAL
8   GLUILEPROVALILEASPVALASPLEUPRO
9   VALTYRALAGLYTHRALAGLUGLUVALLEU
10   GLNGLNGLYALAGLYHISLEUGLUGLYTHR
11   SERLEUPROILEGLYGLYASNSERTHRHIS
12   ALAVALILETHRALAHISTHRGLYLEUPRO
13   THRALALYSMETPHETHRASPLEUTHRLYS
14   LEULYSVALGLYASPLYSPHETYRVALHIS
15   ASNILELYSGLUVALMETALATYRGLNVAL
16   ASPGLNVALLYSVALILEGLUPROTHRASN
17   PHEASPASPLEULEUILEVALPROGLYHIS
18   ASPTYRVALTHRLEULEUTHRCYSTHRPRO
19   TYRMETILEASNTHRHISARGLEULEUVAL
20   ARGGLYHISARGILEPROTYRVALALAGLU
21   VALGLUGLUGLUPHEILEALAALAASNLYS
22   LEUSERHIS

Related Database Links:

PDB 2W1J
GB EJH14048.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks