BMRB Entry 26731

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26731

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Title:
Structural and dynamics studies of Pax5 reveal asymmetry in stability and DNA binding by the Paired domain
Deposition date:
2016-01-14
Original release date:
2016-07-14
Authors:
Perez-Borrajero, Cecilia; McIntosh, Lawrence
Citation:

Citation: Perez-Borrajero, Cecilia; Okon, Mark; McIntosh, Lawrence. "Structural and Dynamics Studies of Pax5 Reveal Asymmetry in Stability and DNA Binding by the Paired Domain"  J. Mol. Biol. 428, 2372-2391 (2016).
PubMed: 27067111

Assembly members:

Assembly members:
Paired_domain_of_Pax5, polymer, 151 residues, Formula weight is not available
DNA_1, polymer, 25 residues, Formula weight is not available
DNA_2, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-MHL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Paired_domain_of_Pax5: GHMDLEKNYPTPRTSRTGHG GVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQL RVSHGCVSKILGRYYETGSI KPGVIGGSKPKVATPKVVEK IAEYKRQNPTMFAWEIRDRL LAERVCDNDTVPSVSSINRI IRTKVQQPPNQ
DNA_1: CGGTGGTCACGCCTCAGTGC CCCAT
DNA_2: GCCACCAGTGCGGAGTCACG GGGTA

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Paired domain of Pax51
2DNA_12
3DNA_23

Entities:

Entity 1, Paired domain of Pax5 151 residues - Formula weight is not available

1   GLYHISMETASPLEUGLULYSASNTYRPRO
2   THRPROARGTHRSERARGTHRGLYHISGLY
3   GLYVALASNGLNLEUGLYGLYVALPHEVAL
4   ASNGLYARGPROLEUPROASPVALVALARG
5   GLNARGILEVALGLULEUALAHISGLNGLY
6   VALARGPROCYSASPILESERARGGLNLEU
7   ARGVALSERHISGLYCYSVALSERLYSILE
8   LEUGLYARGTYRTYRGLUTHRGLYSERILE
9   LYSPROGLYVALILEGLYGLYSERLYSPRO
10   LYSVALALATHRPROLYSVALVALGLULYS
11   ILEALAGLUTYRLYSARGGLNASNPROTHR
12   METPHEALATRPGLUILEARGASPARGLEU
13   LEUALAGLUARGVALCYSASPASNASPTHR
14   VALPROSERVALSERSERILEASNARGILE
15   ILEARGTHRLYSVALGLNGLNPROPROASN
16   GLN

Entity 2, DNA_1 25 residues - Formula weight is not available

1   DCDGDGDTDGDGDTDCDADC
2   DGDCDCDTDCDADGDTDGDC
3   DCDCDCDADT

Entity 3, DNA_2 25 residues - Formula weight is not available

1   DGDCDCDADCDCDADGDTDG
2   DCDGDGDADGDTDCDADCDG
3   DGDGDGDTDA

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