BMRB Entry 26718

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26718

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Title:
Domain Swapped HIV RNase H
Deposition date:
2015-12-11
Original release date:
2016-02-12
Authors:
Mueller, Geoffrey; London, Robert; Zheng, Xunhai
Citation:

Citation: Zheng, Xunhai; Pedersen, Lars; Gabel, Scott; Mueller, Geoffrey; DeRose, Eugene; London, Robert. "Unfolding the HIV-1 reverse transcriptase RNase H domain - how to lose a molecular tug-of-war"  Nucleic Acids Res. 44, 1776-1788 (2016).
PubMed: 26773054

Assembly members:

Assembly members:
RNase_H, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus Human immunodeficiency virus 1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: RHmnel

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNase_H: MNELYQLEKEPIVGAETFYV DGAANRETKLGKAGYVTNRG RQKVVTLTDTTNQKTELQAI YLALQDSGLEVNIVTDSQYA LGIIQAQPDQSESELVNQII EQLIKKEKVYLAWVPAHKGI GGNEQVDKLVSAGIRKVL

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Domain Swapped HIV RNase H1

Entities:

Entity 1, Domain Swapped HIV RNase H 138 residues - Formula weight is not available

Add 422 for correct numbering to HIV-1 Reverse Transcriptase

1   METASNGLULEUTYRGLNLEUGLULYSGLU
2   PROILEVALGLYALAGLUTHRPHETYRVAL
3   ASPGLYALAALAASNARGGLUTHRLYSLEU
4   GLYLYSALAGLYTYRVALTHRASNARGGLY
5   ARGGLNLYSVALVALTHRLEUTHRASPTHR
6   THRASNGLNLYSTHRGLULEUGLNALAILE
7   TYRLEUALALEUGLNASPSERGLYLEUGLU
8   VALASNILEVALTHRASPSERGLNTYRALA
9   LEUGLYILEILEGLNALAGLNPROASPGLN
10   SERGLUSERGLULEUVALASNGLNILEILE
11   GLUGLNLEUILELYSLYSGLULYSVALTYR
12   LEUALATRPVALPROALAHISLYSGLYILE
13   GLYGLYASNGLUGLNVALASPLYSLEUVAL
14   SERALAGLYILEARGLYSVALLEU

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