BMRB Entry 26715

Title:
beta-endorphin
Deposition date:
2015-12-07
Original release date:
2016-07-14
Authors:
Seuring, Carolin; Gath, Julia; Verasdonck, Joeri; Nespovitaya, Nadezhda; Cadalbert, Riccardo; Rivier, Jean; Boeckmann, Anja; Meier, Beat; Riek, Roland
Citation:

Citation: Seuring, Carolin; Gath, Julia; Verasdonck, Joeri; Nespovitaya, Nadezhda; Cadalbert, Riccardo; Rivier, Jean; Boeckmann, Anja; Meier, Beat; Riek, Roland. "Solid-state NMR sequential assignment of the beta-endorphin peptide in its amyloid form"  Biomol. NMR Assign. 10, 259-268 (2016).
PubMed: 27165576

Assembly members:

Assembly members:
beta-endorphin, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-endorphin: YGGFMTSEKSQTPLVTLFKN AIIKNAYKKGE

Data sets:
Data typeCount
13C chemical shifts146
15N chemical shifts32
1H chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta_endorphin1

Entities:

Entity 1, beta_endorphin 31 residues - Formula weight is not available

1   TYRGLYGLYPHEMETTHRSERGLULYSSER
2   GLNTHRPROLEUVALTHRLEUPHELYSASN
3   ALAILEILELYSASNALATYRLYSLYSGLY
4   GLU

Samples:

sample_1: beta-endorphin, [U-100% 13C; U-100% 15N], 25 mg; H2O 10%; D2O 90%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 20ms DARRsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1
3D CCCsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
3D NCOCAsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1

Software:

TOPSPIN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz