BMRB Entry 26660

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26660

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Title:
1H, 13C, and 15N Chemical Shift Assignments for SRC in the lactose-bound state
Deposition date:
2015-09-16
Original release date:
2017-03-03
Authors:
Hemmi, Hikaru
Citation:

Citation: Hemmi, Hikaru; Kuno, Atsushi; Unno, Sachiko; Hirabayashi, Jun. "NMR analysis on the sialic acid-binding mechanism of an R-type lectin mutant by natural evolution-mimicry"  FEBS Lett. 590, 1720-1728 (2016).
PubMed: 27172906

Assembly members:

Assembly members:
src, polymer, 130 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:

Natural source:   Common Name: earthworm   Taxonomy ID: 6398   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lumbricus terrestris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-27b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
src: PKFFYIKSELNGKVLDIGGQ NPAPGSKIITWDQKKGPTAV NQLWYTDQQGVIRSKLNDFA IDASHEQIETQPFDPNNPKR AWIVSGNTIAQLSDRDNVLG VIKSDKGASAHICAWKQHGG PNQKFIIESE

Data sets:
Data typeCount
13C chemical shifts555
15N chemical shifts141
1H chemical shifts901

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1src1
2lactose2

Entities:

Entity 1, src 130 residues - Formula weight is not available

1   PROLYSPHEPHETYRILELYSSERGLULEU
2   ASNGLYLYSVALLEUASPILEGLYGLYGLN
3   ASNPROALAPROGLYSERLYSILEILETHR
4   TRPASPGLNLYSLYSGLYPROTHRALAVAL
5   ASNGLNLEUTRPTYRTHRASPGLNGLNGLY
6   VALILEARGSERLYSLEUASNASPPHEALA
7   ILEASPALASERHISGLUGLNILEGLUTHR
8   GLNPROPHEASPPROASNASNPROLYSARG
9   ALATRPILEVALSERGLYASNTHRILEALA
10   GLNLEUSERASPARGASPASNVALLEUGLY
11   VALILELYSSERASPLYSGLYALASERALA
12   HISILECYSALATRPLYSGLNHISGLYGLY
13   PROASNGLNLYSPHEILEILEGLUSERGLU

Entity 2, lactose - C12 H22 O11 - 342.296 Da.

1   LAT

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks