BMRB Entry 26608

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26608

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the active domains of the type II topoisomerases from Pseudomonas aeruginosa
Deposition date:
2015-07-13
Original release date:
2015-12-18
Authors:
Li, Yan; Ng, Hui Qi; Lee, Michelle Yueqi; Huang, Qiwei; Wong, Ying Lei; Kang, CongBao
Citation:

Citation: Kang, CongBao; Li, Yan; Cherian, Joseph; Liu, Boping; Ng, Hui Qi; Lee, Michelle Yueqi; Poh, Zhi Ying; Wong, Yun Xuan; Huang, Qiwei; Wong, Ying Lei; Hung, Alvin; Hill, Jeffrey; Keller, Thomas H. "Biophysical Studies of Bacterial Topoisomerases Substantiate Their Binding Modes to an Inhibitor"  Biophys. J. 109, 1969-1977 (2015).
PubMed: 26536273

Assembly members:

Assembly members:
Pseudomonas_aeruginosa_active_domains_of_the_type_II_topoisomerases, polymer, 225 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pseudomonas_aeruginosa_active_domains_of_the_type_II_topoisomerases: MATYNADAIEVLSGLDPVRK RPGMYTDTTRPNHLAQEVID NSVDEALAGHAKSVQVILHQ DNSLEVIDDGRGMPVDIHPE EGVPGVELILTKLHAGGKFS NKNYQFSGGLHGVGISVVNA LSTRVEVRVKRDANEYRMTF ADGFKDSDLEVIGTVGKRNT GTSVHFWPDPKYFDSAKFSV SRLKHVLKAKAVLCPGLSVV FEDKNTGERVEWHFEDGLEH HHHHH

Data sets:
Data typeCount
13C chemical shifts607
15N chemical shifts204
1H chemical shifts204

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1active domains of the type II topoisomerases1

Entities:

Entity 1, active domains of the type II topoisomerases 225 residues - Formula weight is not available

1   METALATHRTYRASNALAASPALAILEGLU
2   VALLEUSERGLYLEUASPPROVALARGLYS
3   ARGPROGLYMETTYRTHRASPTHRTHRARG
4   PROASNHISLEUALAGLNGLUVALILEASP
5   ASNSERVALASPGLUALALEUALAGLYHIS
6   ALALYSSERVALGLNVALILELEUHISGLN
7   ASPASNSERLEUGLUVALILEASPASPGLY
8   ARGGLYMETPROVALASPILEHISPROGLU
9   GLUGLYVALPROGLYVALGLULEUILELEU
10   THRLYSLEUHISALAGLYGLYLYSPHESER
11   ASNLYSASNTYRGLNPHESERGLYGLYLEU
12   HISGLYVALGLYILESERVALVALASNALA
13   LEUSERTHRARGVALGLUVALARGVALLYS
14   ARGASPALAASNGLUTYRARGMETTHRPHE
15   ALAASPGLYPHELYSASPSERASPLEUGLU
16   VALILEGLYTHRVALGLYLYSARGASNTHR
17   GLYTHRSERVALHISPHETRPPROASPPRO
18   LYSTYRPHEASPSERALALYSPHESERVAL
19   SERARGLEULYSHISVALLEULYSALALYS
20   ALAVALLEUCYSPROGLYLEUSERVALVAL
21   PHEGLUASPLYSASNTHRGLYGLUARGVAL
22   GLUTRPHISPHEGLUASPGLYLEUGLUHIS
23   HISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks