Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26588

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Please specify the pdb ID.

Name: Backbone chemical shifts of Roquin ROQ domain in complex with a Selex-derived stem-hexa-loop RNA motif
Published: 2016-10-04

Title:

Backbone chemical shifts of Roquin ROQ domain in complex with a Selex-derived stem-hexa-loop RNA motif

Deposition date:

2015-06-16

Original release date:

2016-10-04

Authors:

Schlundt, Andreas; Sattler, Michael

Citation:

Citation: Janowski, Robert; Heinz, Gitta; Schlundt, Andreas; Wommelsdorf, Nina; Brenner, Sven; Gruber, Andreas; Blank, Michael; Buch, Thorsten; Buhmann, Raymund; Zavolan, Mihaela; Niessing, Dierk; Heissmeyer, Vigo; Sattler, Michael. "Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40" Nat. Comm. 7, 11032-11032 (2016).
PubMed: 27010430

Assembly members:

Assembly members:
Roquin_ROQ_domain, polymer, 156 residues, Formula weight is not available
hexa_loop_RNA, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Roquin_ROQ_domain: LQHQNPQQLSSNLWAAVRAR GCQFLGPAMQEEALKLVLLA LEDGSALSRKVLVLFVVQRL EPRFPQASKTSIGHVVQLLY RASCFKVTKRDEDSSLMQLK EEFRTYEALRREHDSQIVQI AMEAGLRIAPDQWSSLLYGD QSHKSHMQSIIDKLQT
hexa_loop_RNA: UGACUGCGUUUUAGGAGUUA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	130
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Roquin ROQ domain	1
2	SELEX consensus hexa-loop RNA	2

Entities:

Entity 1, Roquin ROQ domain 156 residues - Formula weight is not available

ROQ domain

1

LEU

GLN

HIS

GLN

ASN

PRO

GLN

LEU

SER

2

SER

ASN

LEU

TRP

ALA

VAL

ARG

ALA

ARG

3

GLY

CYS

GLN

PHE

LEU

GLY

PRO

ALA

MET

GLN

4

GLU

ALA

LEU

LYS

LEU

VAL

LEU

ALA

5

LEU

GLU

ASP

GLY

SER

ALA

LEU

SER

ARG

LYS

6

VAL

LEU

VAL

LEU

PHE

VAL

GLN

ARG

LEU

7

GLU

PRO

ARG

PHE

PRO

GLN

ALA

SER

LYS

THR

8

SER

ILE

GLY

HIS

VAL

GLN

LEU

TYR

9

ARG

ALA

SER

CYS

PHE

LYS

VAL

THR

LYS

ARG

10

ASP

GLU

ASP

SER

LEU

MET

GLN

LEU

LYS

11

GLU

PHE

ARG

THR

TYR

GLU

ALA

LEU

ARG

12

ARG

GLU

HIS

ASP

SER

GLN

ILE

VAL

GLN

ILE

13

ALA

MET

GLU

ALA

GLY

LEU

ARG

ILE

ALA

PRO

14

ASP

GLN

TRP

SER

LEU

TYR

GLY

ASP

15

GLN

SER

HIS

LYS

SER

HIS

MET

GLN

SER

ILE

16

ILE

ASP

LYS

LEU

GLN

THR

Entity 2, SELEX consensus hexa-loop RNA

Samples:

sample_1: Roquin ROQ domain, [U-100% 15N], 0.20 mM; D2O, [U-100% 2H], 10%; SELEX consensus hexa-loop RNA 0.22 mM; TRIS 20 mM; sodium azide 0.02%; TCEP 4 mM; sodium chloride 150 mM; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR_Analysis v2.4.0, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 26588