BMRB Entry 26549

Name: Backbone 1H, 13C and 15N assignment of the intrinsically disordered region of HCV protein NS5A (191-447)
Published: 2015-04-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26549

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N assignment of the intrinsically disordered region of HCV protein NS5A (191-447)

Deposition date:

2015-03-30

Original release date:

2015-04-27

Authors:

Solyom, Zsofia; Schwarten, Melanie; Willbold, Dieter; Brutscher, Bernhard

Citation:

Citation: Solyom, Zsofia; Ma, Peixiang; Schwarten, Melanie; Durand, Gregory; Willbold, Dieter; Brutscher, Bernhard. "Conformational properties of the disordered region of the NS5A protein from hepatitis C virus and their modulation by SH3 binding and phosphorylation" Biophys J. 109, 1483-1496 (2015).
PubMed: 6445449

Assembly members:

Assembly members:
NS5A(191-447), polymer, 268 residues, Formula weight is not available

Natural source:

Natural source: Common Name: hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus hepatitis C virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS5A(191-447): GHMASGSLRGGEPEPDVTVL TSMLTDPSHITAETAKRRLA RGSPPSLASSSASQLSAPSL KATCTTHHDSPDADLIEANL LWRQEMGGNITRVESENKVV ILDSFEPLHADGDEREISVA AEILRKSRKFPSALPIWARP DYNPPLLESWKDPDYVPPVV HGCPLPPTKAPPIPPPRRKR TVVLTESNVSSALAELATKT FGSSGSSAVDSGTATALPDQ ASDDGDKGSDVESYSSMPPL EGEPGDPDLSDGSWSTVSEE ASEDVVCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	689
15N chemical shifts	235
1H chemical shifts	433

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS5A D2D3	1

Entities:

Entity 1, NS5A D2D3 268 residues - Formula weight is not available

1

GLY

HIS

MET

ALA

SER

GLY

SER

LEU

ARG

GLY

2

GLY

GLU

PRO

GLU

PRO

ASP

VAL

THR

VAL

LEU

3

THR

SER

MET

LEU

THR

ASP

PRO

SER

HIS

ILE

4

THR

ALA

GLU

THR

ALA

LYS

ARG

LEU

ALA

5

ARG

GLY

SER

PRO

SER

LEU

ALA

SER

6

SER

ALA

SER

GLN

LEU

SER

ALA

PRO

SER

LEU

7

LYS

ALA

THR

CYS

THR

HIS

ASP

SER

8

PRO

ASP

ALA

ASP

LEU

ILE

GLU

ALA

ASN

LEU

9

LEU

TRP

ARG

GLN

GLU

MET

GLY

ASN

ILE

10

THR

ARG

VAL

GLU

SER

GLU

ASN

LYS

VAL

11

ILE

LEU

ASP

SER

PHE

GLU

PRO

LEU

HIS

ALA

12

ASP

GLY

ASP

GLU

ARG

GLU

ILE

SER

VAL

ALA

13

ALA

GLU

ILE

LEU

ARG

LYS

SER

ARG

LYS

PHE

14

PRO

SER

ALA

LEU

PRO

ILE

TRP

ALA

ARG

PRO

15

ASP

TYR

ASN

PRO

LEU

GLU

SER

TRP

16

LYS

ASP

PRO

ASP

TYR

VAL

PRO

VAL

17

HIS

GLY

CYS

PRO

LEU

PRO

THR

LYS

ALA

18

PRO

ILE

PRO

ARG

LYS

ARG

19

THR

VAL

LEU

THR

GLU

SER

ASN

VAL

SER

20

SER

ALA

LEU

ALA

GLU

LEU

ALA

THR

LYS

THR

21

PHE

GLY

SER

GLY

SER

ALA

VAL

ASP

22

SER

GLY

THR

ALA

THR

ALA

LEU

PRO

ASP

GLN

23

ALA

SER

ASP

GLY

ASP

LYS

GLY

SER

ASP

24

VAL

GLU

SER

TYR

SER

MET

PRO

LEU

25

GLU

GLY

GLU

PRO

GLY

ASP

PRO

ASP

LEU

SER

26

ASP

GLY

SER

TRP

SER

THR

VAL

SER

GLU

27

ALA

SER

GLU

ASP

VAL

CYS

Samples:

sample_1: NS5A(191-447), [U-100% 13C; U-100% 15N], 120 uM; D2O, [U-100% 2H], 10%; potassium phosphate 50 mM; sodium chloride 20 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.32 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N BEST-TROSY	sample_1	isotropic	sample_conditions_1
3D BT-HNCOCACB	sample_1	isotropic	sample_conditions_1
3D BT-iHNCACB	sample_1	isotropic	sample_conditions_1
3D BT-hNcocaNH	sample_1	isotropic	sample_conditions_1
3D BT-hnCOcaNH	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks