BMRB Entry 26541

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26541

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Title:
Backbone Chemical Shift Assignment of the FimH lectin domain from Escherichia coli
Deposition date:
2015-03-13
Original release date:
2015-05-11
Authors:
Fiege, Brigitte; Ernst, Beat
Citation:

Citation: Fiege, Brigitte; Rabbani, Said; Preston, Roland; Jakob, Roman; Zihlmann, Pascal; Schwardt, Oliver; Jiang, Xiaohua; Maier, Timm; Ernst, Beat. "he Tyrosine Gate of the Bacterial Lectin FimH: A Conformational Analysis by NMR Spectroscopy and X-ray Crystallography"  Chembiochem 16, 1235-1246 (2015).
PubMed: 25940742

Assembly members:

Assembly members:
FimH-CRD, polymer, 173 residues, 18643.8386 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDsbA3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FimH-CRD: FACKTANGTAIPIGGGSANV YVNLAPVVNVGQNLVVDLST QIFCHNDYPETITDYVTLQR GSAYGGVLSNFSGTVKYSGS SYPFPTTSETPRVVYNSRTD KPWPVALYLTPVSSAGGVAI KAGSLIAVLILRQTNNYNSD DFQFVWNIYANNDVVVPTGL VPRGSLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts173
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FimH-CRD1

Entities:

Entity 1, FimH-CRD 173 residues - 18643.8386 Da.

1   PHEALACYSLYSTHRALAASNGLYTHRALA
2   ILEPROILEGLYGLYGLYSERALAASNVAL
3   TYRVALASNLEUALAPROVALVALASNVAL
4   GLYGLNASNLEUVALVALASPLEUSERTHR
5   GLNILEPHECYSHISASNASPTYRPROGLU
6   THRILETHRASPTYRVALTHRLEUGLNARG
7   GLYSERALATYRGLYGLYVALLEUSERASN
8   PHESERGLYTHRVALLYSTYRSERGLYSER
9   SERTYRPROPHEPROTHRTHRSERGLUTHR
10   PROARGVALVALTYRASNSERARGTHRASP
11   LYSPROTRPPROVALALALEUTYRLEUTHR
12   PROVALSERSERALAGLYGLYVALALAILE
13   LYSALAGLYSERLEUILEALAVALLEUILE
14   LEUARGGLNTHRASNASNTYRASNSERASP
15   ASPPHEGLNPHEVALTRPASNILETYRALA
16   ASNASNASPVALVALVALPROTHRGLYLEU
17   VALPROARGGLYSERLEUGLUHISHISHIS
18   HISHISHIS

Related Database Links:

UniProt A2IC68_ECOLX
PDB 4BUQ 4CST 1UWF 1TR7 3MCY 4CSS 4AV5 4AV4 2VCO 1KLF 4AUU 1QUN 4CA4 4LOV 3ZPD 4X50 4X5Q 4X5R 4X5P
BMRB 19066
GB CAM92099.1
AlphaFold A2IC68

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks