BMRB Entry 26534

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26534

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Title:
Backbone and side chain 1H, 13C, and 15N chemical shift assignments for Extracellular Loop 1 (ECL1) of FtsX in Streptococcus pneumoniae
Deposition date:
2015-03-09
Original release date:
2016-01-06
Authors:
Fu, Yue; Giedroc, David
Citation:

Citation: Fu, Yue; Bruce, Kevin; Rued, Britta; Winkler, Malcolm; Giedroc, David. "1H, 13C, 15N resonance assignments of the extracellular loop 1 domain (ECL1) of Streptococcus pneumoniae D39 FtsX, an essential cell division protein"  Biomol. NMR Assign. 10, 89-92 (2016).
PubMed: 26370567

Assembly members:

Assembly members:
FtsXECL1, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 373153   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FtsXECL1: GAMATAKLATDIENNVRVVV YIRKDVEDNSQTIEKEGQTV TNNDYHKVYDSLKNMSTVKS VTFSSKEEQYEKLTEIMGDN WKIFEGDANPLYDAYIVEAN APNDVKTIAEDAKKIEGVSE VQDGGA

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts122
1H chemical shifts786

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FtsXECL1 monomer1

Entities:

Entity 1, FtsXECL1 monomer 126 residues - Formula weight is not available

Residue 1-4 represent a cloning artefact from His6-affinity tag after TEV cleavage. This is the bigger of the two extracellular loops of a membrane protein FtsX.

1   GLYALAMETALATHRALALYSLEUALATHR
2   ASPILEGLUASNASNVALARGVALVALVAL
3   TYRILEARGLYSASPVALGLUASPASNSER
4   GLNTHRILEGLULYSGLUGLYGLNTHRVAL
5   THRASNASNASPTYRHISLYSVALTYRASP
6   SERLEULYSASNMETSERTHRVALLYSSER
7   VALTHRPHESERSERLYSGLUGLUGLNTYR
8   GLULYSLEUTHRGLUILEMETGLYASPASN
9   TRPLYSILEPHEGLUGLYASPALAASNPRO
10   LEUTYRASPALATYRILEVALGLUALAASN
11   ALAPROASNASPVALLYSTHRILEALAGLU
12   ASPALALYSLYSILEGLUGLYVALSERGLU
13   VALGLNASPGLYGLYALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks