BMRB Entry 26510

Title:
The Sortase A Enzyme That Attaches Proteins to the Cell Wall of Bacillus anthracis Contains an Unusual Active Site Architecture
Deposition date:
2015-02-16
Original release date:
2015-02-25
Authors:
Weiner, Ethan; Robson, Scott; Marohn, Melanie; Clubb, Robert
Citation:

Citation: Weiner, Ethan; Robson, Scott; Marohn, Melanie; Clubb, Robert. "The Sortase A Enzyme That Attaches Proteins to the Cell Wall of Bacillus anthracis Contains an Unusual Active Site Architecture"  J. Biol. Chem. 285, 23433-23443 (2010).
PubMed: 20489200

Assembly members:

Assembly members:
Ba-SrtA, polymer, 210 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: anthrax   Taxonomy ID: 1392   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus anthracis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Data sets:
Data typeCount
order parameters91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ba-SrtA1

Entities:

Entity 1, Ba-SrtA 210 residues - Formula weight is not available

1   METASNLYSGLNARGILETYRSERILEVAL
2   ALAILELEULEUPHEVALVALGLYGLYVAL
3   LEUILEGLYLYSPROPHETYRASPGLYTYR
4   GLNALAGLULYSLYSGLNTHRGLUASNVAL
5   GLNALAVALGLNLYSMETASPTYRGLULYS
6   HISGLUTHRGLUPHEVALASPALASERLYS
7   ILEASPGLNPROASPLEUALAGLUVALALA
8   ASNALASERLEUASPLYSLYSGLNVALILE
9   GLYARGILESERILEPROSERVALSERLEU
10   GLULEUPROVALLEULYSSERSERTHRGLU
11   LYSASNLEULEUSERGLYALAALATHRVAL
12   LYSGLUASNGLNVALMETGLYLYSGLYASN
13   TYRALALEUALAGLYHISASNMETSERLYS
14   LYSGLYVALLEUPHESERASPILEALASER
15   LEULYSLYSGLYASPLYSILETYRLEUTYR
16   ASPASNGLUASNGLUTYRGLUTYRALAVAL
17   THRGLYVALSERGLUVALTHRPROASPLYS
18   TRPGLUVALVALGLUASPHISGLYLYSASP
19   GLUILETHRLEUILETHRCYSVALSERVAL
20   LYSASPASNSERLYSARGTYRVALVALALA
21   GLYASPLEUVALGLYTHRLYSALALYSLYS

Samples:

sample_1: Ba-SrtA, [U-100% 15N], 4 mM; MES 10 mM; BisTris 20 mM; D2O 7%; H2O 93%

sample_2: Ba-SrtA, [U-100% 13C; U-100% 15N], 2.5 mM; MES 10 mM; BisTris 20 mM; D2O 7%; H2O 93%

sample_3: Ba-SrtA, [U-100% 13C; U-100% 15N], 2.5 mM; MES 10 mM; BisTris 20 mM; D2O 100%

sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC 1sample_1isotropicsample_conditions_1
2D 1H-15N HSQC 2sample_2isotropicsample_conditions_1
2D 1H-15N HSQC 3sample_3isotropicsample_conditions_1
15N-{1H} NOE 1sample_1isotropicsample_conditions_1
15N-{1H} NOE 2sample_2isotropicsample_conditions_1
15N-{1H} NOE 3sample_3isotropicsample_conditions_1

Software:

Modelfree v4.20, Palmer - Model-free analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

CARA, Keller and Wuthrich - data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11570 16811
PDB
DBJ BAL16456 BAR78533 BAR86244 GAE96182 GAO57723
EMBL CCW04836 CDN34078 CEY33381 CGF95881 CGG53955
GB AAP07696 AAP24701 AAS39688 AAT29792 AAT52982
REF NP_830495 NP_843215 WP_001041406 WP_001041710 WP_001041711