BMRB Entry 25955

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PDB ID: 5frf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25955
MolProbity Validation Chart

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Title:
Solution structure of reduced and zinc-bound RsrA
Deposition date:
2016-01-15
Original release date:
2016-08-01
Authors:
Zdanowski, K.; Pecqueur, L.; Werner, J.; Potts, Jennifer; Kleanthous, C.
Citation:

Citation: Rajasekar, K.; Zdanowski, K.; Yan, J.; Hopper, J.; Francis, M.; Seepersad, C.; Sharp, C.; Pecqueur, L.; Werner, J.; Robinson, C.; Mohammed, S.; Potts, Jennifer; Kleanthous, C.. "The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core"  Nat. Commun. 7, 12194-12194 (2016).
PubMed: 27432510

Assembly members:

Assembly members:
ANTI-SIGMA_FACTOR_RSRA, polymer, 108 residues, 11807.9444 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Streptomyces coelicolor   Taxonomy ID: 1902   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces coelicolor

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ANTI-SIGMA_FACTOR_RSRA: GSHMSAGEPHETDCSEILDH LYEFLDKEMPDSDAVKFEHH FEESSPCLEKYGLEQAVKKL VKRAAGQDDVPGDLRAKVMG RLDLIRSGQSVPEHDVAAAP SSSAPQES

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts93
1H chemical shifts598

Additional metadata:

  • Assembly
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ANTI-SIGMA FACTOR RSRA1
2ZINC ION2

Entities:

Entity 1, ANTI-SIGMA FACTOR RSRA 108 residues - 11807.9444 Da.

1   GLYSERHISMETSERALAGLYGLUPROHIS
2   GLUTHRASPCYSSERGLUILELEUASPHIS
3   LEUTYRGLUPHELEUASPLYSGLUMETPRO
4   ASPSERASPALAVALLYSPHEGLUHISHIS
5   PHEGLUGLUSERSERPROCYSLEUGLULYS
6   TYRGLYLEUGLUGLNALAVALLYSLYSLEU
7   VALLYSARGALAALAGLYGLNASPASPVAL
8   PROGLYASPLEUARGALALYSVALMETGLY
9   ARGLEUASPLEUILEARGSERGLYGLNSER
10   VALPROGLUHISASPVALALAALAALAPRO
11   SERSERSERALAPROGLNGLUSER

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

UNP Q9RL96 RSRA_STRCO
AlphaFold Q9RL96

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