BMRB Entry 25927

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PDB ID: 2na4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25927
MolProbity Validation Chart

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Title:
Curli secretion specificity factor CsgE W48A/F79A mutant
Deposition date:
2015-12-21
Original release date:
2016-06-13
Authors:
Shu, Qin; Krezel, Andrzej; Frieden, Carl
Citation:

Citation: Shu, Qin; Krezel, Andrzej; Hultgren, Scott; Frieden, Carl. "Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation"  Proc. Natl. Acad. Sci. U.S.A. 113, 7130-7135 (2016).
PubMed: 27298344

Assembly members:

Assembly members:
entity, polymer, 108 residues, 13021.44 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: AVEVEVPGLLTDHTVSSIGH DFYRAFSDKWESDYTGNLTI NERPSARAGSWITITVNQDV IFQTFLFPLKRDFEKTVVAA LIQTEEALNRRQINQALLST GDLAHDEF

Data sets:
Data typeCount
13C chemical shifts470
15N chemical shifts116
1H chemical shifts747

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 108 residues - 13021.44 Da.

1   ALAVALGLUVALGLUVALPROGLYLEULEU
2   THRASPHISTHRVALSERSERILEGLYHIS
3   ASPPHETYRARGALAPHESERASPLYSTRP
4   GLUSERASPTYRTHRGLYASNLEUTHRILE
5   ASNGLUARGPROSERALAARGALAGLYSER
6   TRPILETHRILETHRVALASNGLNASPVAL
7   ILEPHEGLNTHRPHELEUPHEPROLEULYS
8   ARGASPPHEGLULYSTHRVALVALALAALA
9   LEUILEGLNTHRGLUGLUALALEUASNARG
10   ARGGLNILEASNGLNALALEULEUSERTHR
11   GLYASPLEUALAHISASPGLUPHE

Related Database Links:

UNP P0AE95
GB ECK1025 EcoGene:EG13411
NCBI 945711
AlphaFold P52105

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks