BMRB Entry 25923
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PDB ID: 2na1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25923
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gray, Felicia; Cho, Hyo Je; Shulka, Shirish; He, Shihan; Harris, Ashley; Boytsov, Bohdan; Jaremko, Lukasz; Jaremko, Mariusz; Demeler, Borries; Lawlor, Elizabeth; Grembecka, Jolanta; Cierpicki, Tomasz. "BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization" Nat. Commun. 9, 13343-13343 (2016).
PubMed: 27827373
Assembly members:
entity, polymer, 155 residues, 17139.982 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32p
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 469 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 553 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 155 residues - 17139.982 Da.
Residues 1-5 are cloning artifact. Sequence contains fusion of PHC2_B residues 30-64 directly to N-terminus of BMI1 residues 121-235.
| 1 | GLY | PRO | ALA | MET | GLY | LYS | PRO | GLN | ILE | LEU | ||||
| 2 | THR | HIS | VAL | ILE | GLU | GLY | PHE | VAL | ILE | GLN | ||||
| 3 | GLU | GLY | ALA | GLU | PRO | PHE | PRO | VAL | GLY | ARG | ||||
| 4 | SER | SER | LEU | LEU | VAL | GLY | ASN | LEU | LYS | GLY | ||||
| 5 | ASP | LYS | ARG | ILE | ILE | THR | ASP | ASP | GLU | ILE | ||||
| 6 | ILE | SER | LEU | SER | ILE | GLU | PHE | PHE | ASP | GLN | ||||
| 7 | ASN | ARG | LEU | ASP | ARG | LYS | VAL | ASN | LYS | ASP | ||||
| 8 | LYS | GLU | LYS | SER | LYS | GLU | GLU | VAL | ASN | ASP | ||||
| 9 | LYS | ARG | TYR | LEU | ARG | CYS | PRO | ALA | ALA | MET | ||||
| 10 | THR | VAL | MET | HIS | LEU | ARG | LYS | PHE | LEU | ARG | ||||
| 11 | SER | LYS | MET | ASP | ILE | PRO | ASN | THR | PHE | GLN | ||||
| 12 | ILE | ASP | VAL | MET | TYR | GLU | GLU | GLU | PRO | LEU | ||||
| 13 | LYS | ASP | TYR | TYR | THR | LEU | MET | ASP | ILE | ALA | ||||
| 14 | TYR | ILE | TYR | THR | TRP | ARG | ARG | ASN | GLY | PRO | ||||
| 15 | LEU | PRO | LEU | LYS | TYR | ARG | VAL | ARG | PRO | THR | ||||
| 16 | CYS | LYS | ARG | MET | LYS |
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