BMRB Entry 25921

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25921

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Title:
NMR assignment of the 65-residue-long inactivating factor of glutanmine synthetase I from cyanobacterirum Synechocystis sp. PCC 6803
Deposition date:
2015-12-15
Original release date:
2016-06-13
Authors:
Pantoja-Uceda, David; Neira, Jose; Santoro, Jorge
Citation:

Citation: Pantoja-Uceda, David; Neira, Jose; Saelices, Lorena; Robles-Rengel, Rocio; Florencio, Francsico; Muro-Pastor, M. Isabel; Santoro, Jorge. "Dissecting the Binding between Glutamine Synthetase and Its Two Natively Unfolded Protein Inhibitors"  Biochemistry 55, 3370-3382 (2016).
PubMed: 27232663

Assembly members:

Assembly members:
IF7, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 1148   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Synechocystis sp. PPC 6803

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E.coli (BL21)   Vector: pet24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IF7: MGSSHHHHHHSSGLVPRGSH MSTQQQARALMMRHHQFIKN RQQSMLSRAAAEIGVEAEKD FWTTVQGKPQSSFRTTYDRS NASLS

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts74
1H chemical shifts272

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IF71

Entities:

Entity 1, IF7 85 residues - Formula weight is not available

Residues 1-20 are not presente in native IF7 renumbering -20 numbers

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERTHRGLNGLNGLNALAARGALALEU
4   METMETARGHISHISGLNPHEILELYSASN
5   ARGGLNGLNSERMETLEUSERARGALAALA
6   ALAGLUILEGLYVALGLUALAGLULYSASP
7   PHETRPTHRTHRVALGLNGLYLYSPROGLN
8   SERSERPHEARGTHRTHRTYRASPARGSER
9   ASNALASERLEUSER

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