BMRB Entry 25806

Title:
NMR structure of the prolactin receptor transmembrane domain
Deposition date:
2015-09-11
Original release date:
2016-05-23
Authors:
Bugge, Katrine; Kragelund, Birthe
Citation:

Citation: Bugge, Katrine; Papaleo, Elena; Haxholm, Gitte; Hopper, Jonathan; Dagil, Robert; Robinson, Carol; Olsen, Johan; Lindorff-Larsen, Kresten; Kragelund, Birthe. "A combined computational and structural model of the full-length human prolactin receptor"  Nat. Commun. 7, 11578-11578 (2016).
PubMed: 27174498

Assembly members:

Assembly members:
PRLRTMD, polymer, 37 residues, 3960.773 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRLRTMD: GSFTMNDTTVWISVAVLSAV ICLIIVWAVALKGYSMV

Data sets:
Data typeCount
13C chemical shifts161
15N chemical shifts37
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prolactin receptor transmembrane domain1

Entities:

Entity 1, prolactin receptor transmembrane domain 37 residues - 3960.773 Da.

Residues 204-205 represent non-native residues left over from thrombin digestion. This is the transmembrane domain of a receptor

1   GLYSERPHETHRMETASNASPTHRTHRVAL
2   TRPILESERVALALAVALLEUSERALAVAL
3   ILECYSLEUILEILEVALTRPALAVALALA
4   LEULYSGLYTYRSERMETVAL

Samples:

sample_1: PRLRTMD, [U-13C; U-15N], 0.8 mM; DSS 2 mM; sodium azide 0.05%; DHPC 560 mM; sodium phosphate 20 mM; TCEP 4 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: PRLRTMD, [U-13C; U-15N], 1 mM; DSS 2 mM; sodium azide 0.05%; DHPC, [U-2H], 700 mM; sodium phosphate 20 mM; TCEP 4 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample15N: PRLRTMD, [U-15N], 0.7 mM; DSS 2 mM; sodium azide 0.05%; DHPC 490 mM; sodium phosphate 20 mM; TCEP 4 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNHAsample15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1

Software:

ARIA v2.3.2, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 750 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks