BMRB Entry 25770

Assembly:

Entities:

Entity 1, TEN 159 residues - Formula weight is not available

Samples:

sample_1: TEN, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.02 % w/v; H2O 90%; D2O, [U-2H], 10%

sample_2: TEN, [U-99% 15N], 0.8 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.02 % w/v; H2O 90%; D2O, [U-2H], 10%

sample_3: TEN, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.02 % w/v; D2O, [U-2H], 100%

sample_4: TEN 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.02 % w/v; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMRest, Vladimir Polshakov - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

• Bruker Avance 700 MHz
• Bruker Avance 600 MHz