BMRB Entry 25752
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25752
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Citation: Sinha, Kaustubh; Sangani, Sahil; Kehr, Andrew; Rule, Gordon; Jen-Jacobson, Linda. "Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex.'" Biochemistry 55, 6115-6132 (2016).
PubMed: 27786446
Assembly members:
EcoRV, polymer, 244 residues, 28650 Da.
5'-GCAAAGATATCTTTCG-3', polymer, 16 residues, 9760 Da.
LUTETIUM (III) ION, non-polymer, 174.967 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 22b+
Entity Sequences (FASTA):
EcoRV: SLRSDLINALYDENQKYDVC
GIISAEGKIYPLGSDTKVLS
TIFELFSRPIINKIAEKHGY
IVEEPKQQNHYPDFTLYKPS
EPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNI
VYPFDQYIAHWIIGYVYTRV
ATRKSSLKTYNINELNEIPK
PYKGVKVFLQDKWVIAGDLA
GSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYER
TSQLRNDKYNNISEYRNWIY
RGRK
5'-GCAAAGATATCTTTCG-3': GCAAAGATATCTTTCG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 371 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 291 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RV dimer, 1 | 1 |
| 2 | RV dimer, 2 | 1 |
| 3 | AAA DNA fragment, 1 | 2 |
| 4 | AAA DNA fragment, 2 | 2 |
| 5 | Lutetium ion, 1 | 3 |
| 6 | Lutetium ion, 2 | 3 |
| 7 | Lutetium ion, 3 | 3 |
Entities:
Entity 1, RV dimer, 1 244 residues - 28650 Da.
| 1 | SER | LEU | ARG | SER | ASP | LEU | ILE | ASN | ALA | LEU | ||||
| 2 | TYR | ASP | GLU | ASN | GLN | LYS | TYR | ASP | VAL | CYS | ||||
| 3 | GLY | ILE | ILE | SER | ALA | GLU | GLY | LYS | ILE | TYR | ||||
| 4 | PRO | LEU | GLY | SER | ASP | THR | LYS | VAL | LEU | SER | ||||
| 5 | THR | ILE | PHE | GLU | LEU | PHE | SER | ARG | PRO | ILE | ||||
| 6 | ILE | ASN | LYS | ILE | ALA | GLU | LYS | HIS | GLY | TYR | ||||
| 7 | ILE | VAL | GLU | GLU | PRO | LYS | GLN | GLN | ASN | HIS | ||||
| 8 | TYR | PRO | ASP | PHE | THR | LEU | TYR | LYS | PRO | SER | ||||
| 9 | GLU | PRO | ASN | LYS | LYS | ILE | ALA | ILE | ASP | ILE | ||||
| 10 | LYS | THR | THR | TYR | THR | ASN | LYS | GLU | ASN | GLU | ||||
| 11 | LYS | ILE | LYS | PHE | THR | LEU | GLY | GLY | TYR | THR | ||||
| 12 | SER | PHE | ILE | ARG | ASN | ASN | THR | LYS | ASN | ILE | ||||
| 13 | VAL | TYR | PRO | PHE | ASP | GLN | TYR | ILE | ALA | HIS | ||||
| 14 | TRP | ILE | ILE | GLY | TYR | VAL | TYR | THR | ARG | VAL | ||||
| 15 | ALA | THR | ARG | LYS | SER | SER | LEU | LYS | THR | TYR | ||||
| 16 | ASN | ILE | ASN | GLU | LEU | ASN | GLU | ILE | PRO | LYS | ||||
| 17 | PRO | TYR | LYS | GLY | VAL | LYS | VAL | PHE | LEU | GLN | ||||
| 18 | ASP | LYS | TRP | VAL | ILE | ALA | GLY | ASP | LEU | ALA | ||||
| 19 | GLY | SER | GLY | ASN | THR | THR | ASN | ILE | GLY | SER | ||||
| 20 | ILE | HIS | ALA | HIS | TYR | LYS | ASP | PHE | VAL | GLU | ||||
| 21 | GLY | LYS | GLY | ILE | PHE | ASP | SER | GLU | ASP | GLU | ||||
| 22 | PHE | LEU | ASP | TYR | TRP | ARG | ASN | TYR | GLU | ARG | ||||
| 23 | THR | SER | GLN | LEU | ARG | ASN | ASP | LYS | TYR | ASN | ||||
| 24 | ASN | ILE | SER | GLU | TYR | ARG | ASN | TRP | ILE | TYR | ||||
| 25 | ARG | GLY | ARG | LYS |
Entity 2, AAA DNA fragment, 1 16 residues - 9760 Da.
| 1 | DG | DC | DA | DA | DA | DG | DA | DT | DA | DT | ||||
| 2 | DC | DT | DT | DT | DC | DG |
Entity 3, Lutetium ion, 1 - Lu - 174.967 Da.
| 1 | LU |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks