BMRB Entry 25727

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25727

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Title:
1H, 13C, and 15N Chemical Shift Assignments for Histamine-Binding Protein (D24R) bound to histamine
Deposition date:
2015-07-27
Original release date:
2017-06-27
Authors:
Lim, Jackwee; Valentine, Kathleen; Kasinath, Vignesh; Harpole, Kyle; Sharp, Kim; Wand, Joshua
Citation:

Citation: Caro, Jose; Harpole, Kyle; Kasinath, Vignesh; Lim, Jackwee; Granja, Jeffrey; Valentine, Kathleen; Sharp, Kim; Wand, A Joshua. "Entropy in molecular recognition by proteins"  Proc. Natl. Acad. Sci. U.S.A. 114, 6563-6568 (2017).
PubMed: 28584100

Assembly members:

Assembly members:
HBP(d24r), polymer, 173 residues, Formula weight is not available
HISTAMINE, non-polymer, 111.145 Da.

Natural source:

Natural source:   Common Name: mites and ticks   Taxonomy ID: 34631   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rhipicephalus appendiculatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HBP(d24r): GSNQPDWADEAANGAHQDAW KSLKARVENVYYMVKATYKN DPVWGNDFTCVGVMANDVNE DEKSIQAEFLFMNNADTNMQ FATEKVTAVKMYGYNRENAF RYETEDGQVFTDVIAYSDDN CDVIYVPGTDGNEEGYELWT TDYDNIPANCLNKFNEYAVG RETRDVFTSACLE

Data sets:
Data typeCount
13C chemical shifts381
15N chemical shifts160
1H chemical shifts367
order parameters210

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HBPd24r1
2histamine2

Entities:

Entity 1, HBPd24r 173 residues - Formula weight is not available

1   GLYSERASNGLNPROASPTRPALAASPGLU
2   ALAALAASNGLYALAHISGLNASPALATRP
3   LYSSERLEULYSALAARGVALGLUASNVAL
4   TYRTYRMETVALLYSALATHRTYRLYSASN
5   ASPPROVALTRPGLYASNASPPHETHRCYS
6   VALGLYVALMETALAASNASPVALASNGLU
7   ASPGLULYSSERILEGLNALAGLUPHELEU
8   PHEMETASNASNALAASPTHRASNMETGLN
9   PHEALATHRGLULYSVALTHRALAVALLYS
10   METTYRGLYTYRASNARGGLUASNALAPHE
11   ARGTYRGLUTHRGLUASPGLYGLNVALPHE
12   THRASPVALILEALATYRSERASPASPASN
13   CYSASPVALILETYRVALPROGLYTHRASP
14   GLYASNGLUGLUGLYTYRGLULEUTRPTHR
15   THRASPTYRASPASNILEPROALAASNCYS
16   LEUASNLYSPHEASNGLUTYRALAVALGLY
17   ARGGLUTHRARGASPVALPHETHRSERALA
18   CYSLEUGLU

Entity 2, histamine - C5 H9 N3 - 111.145 Da.

1   HSM

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