BMRB Entry 25717

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PDB ID: 2n5i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25717
MolProbity Validation Chart

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Title:
PltL-pyrrolyl
Deposition date:
2015-07-17
Original release date:
2015-09-16
Authors:
Jaremko, Matt; Lee, D.; Burkart, Michael
Citation:

Citation: Jaremko, Matt; Lee, D.; Opella, Stanley; Burkart, Michael. "Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL"  J. Am. Chem. Soc. 137, 11546-11549 (2015).
PubMed: 26340431

Assembly members:

Assembly members:
entity, polymer, 90 residues, 10562.150 Da.
entity_4EM, non-polymer, 434.381 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 220664   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MDGEEVKEKIRRYIMEDLIG PSAKEDELDDQTPLLEWGIL NSMNIVKLMVYIRDEMGVSI PSTHITGKYFKDLNAISRTV EQLKAESALE

Data sets:
Data typeCount
13C chemical shifts313
15N chemical shifts90
1H chemical shifts686

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1
2entity_4EM2

Entities:

Entity 1, entity 90 residues - 10562.150 Da.

1   METASPGLYGLUGLUVALLYSGLULYSILE
2   ARGARGTYRILEMETGLUASPLEUILEGLY
3   PROSERALALYSGLUASPGLULEUASPASP
4   GLNTHRPROLEULEUGLUTRPGLYILELEU
5   ASNSERMETASNILEVALLYSLEUMETVAL
6   TYRILEARGASPGLUMETGLYVALSERILE
7   PROSERTHRHISILETHRGLYLYSTYRPHE
8   LYSASPLEUASNALAILESERARGTHRVAL
9   GLUGLNLEULYSALAGLUSERALALEUGLU

Entity 2, entity_4EM - C16 H27 N4 O8 P - 434.381 Da.

1   4EM

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