BMRB Entry 25706

Click here to enlarge.

PDB ID: 2n5d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25706
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C, and 15N Chemical Shift Assignments of PKS domains
Deposition date:
2015-07-14
Original release date:
2016-03-21
Authors:
Dorival, Gonathan; Annaval, Thibault; Risser, Fanny; Collin, Sabrina; Roblin, Pierre; Jacob, Christophe; Gruez, Arnaud; Chagot, Benjamin; Weissman, Kira
Citation:

Citation: Dorival, Gonathan; Annaval, Thibault; Risser, Fanny; Collin, Sabrina; Roblin, Pierre; Jacob, Christophe; Gruez, Arnaud; Chagot, Benjamin; Weissman, Kira. "Characterization of Intersubunit Communication in the Virginiamycin trans-Acyl Transferase Polyketide Synthase"  J. Am. Chem. Soc. 138, 4155-4167 (2016).
PubMed: 26982529

Assembly members:

Assembly members:
entity, polymer, 84 residues, 8455.489 Da.

Natural source:

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 1961   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces virginiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBG102 (pet27 derivative)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPGSYTGAGEPSQADLDALL SAVRDNRLSIEQAVTLLTPR RGGGSGGGSMDAKEILTRFK DGGLDRAAAQALLAGRTPAA APRP

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts80
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 84 residues - 8455.489 Da.

Residues 1-5 represent a non-native affinity tag. Residues 6-41 is from VirA protein. Residues 42-49 is an non-native fusion linker. Residues 50-88 is from VirFG protein.

1   GLYPROGLYSERTYRTHRGLYALAGLYGLU
2   PROSERGLNALAASPLEUASPALALEULEU
3   SERALAVALARGASPASNARGLEUSERILE
4   GLUGLNALAVALTHRLEULEUTHRPROARG
5   ARGGLYGLYGLYSERGLYGLYGLYSERMET
6   ASPALALYSGLUILELEUTHRARGPHELYS
7   ASPGLYGLYLEUASPARGALAALAALAGLN
8   ALALEULEUALAGLYARGTHRPROALAALA
9   ALAPROARGPRO

Related Database Links:

GB BAF50727.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks