BMRB Entry 25692

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PDB ID: 2n53
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25692
MolProbity Validation Chart

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Title:
SOLUTION STRUCTURE OF OVIS ARIES PRP
Deposition date:
2015-07-06
Original release date:
2016-05-23
Authors:
Munoz, Carola; Egalon, Angelique; Beringue, Vincent; Rezaei, Human; Dron, Michel; Sizun, Christina
Citation:

Citation: Munoz-Montesino, Carola; Sizun, Christina; Moudjou, Mohammed; Herzog, Laetitia; Reine, Fabienne; Chapuis, Jerome; Ciric, Danica; Igel-Egalon, Angelique; Laude, Hubert; Beringue, Vincent; Rezaei, Human; Dron, Michel. "Generating Bona Fide Mammalian Prions with Internal Deletions"  J. Virol. 90, 6963-6975 (2016).
PubMed: 27226369

Assembly members:

Assembly members:
entity, polymer, 206 residues, 16263.355 Da.

Natural source:

Natural source:   Common Name: sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MGSSHHHHHHSSGLVPRGSH MSNKPSKPKTNMKHVAGAAA AGAVVGGLGGYMLGSVMSRP LIHFGNDYEDRYYRENMYRY PNQVYYRPVDQYSNQNNFVH DCVNITVKQHTVTTTTKGEN FTETDIKIMERVVEQMCITQ YQRESQAYYQRGASVNITVK QHTVTTTTKGENFTETDIKI MERVVEQMCITQYQRESQAY YQRGAS

Data sets:
Data typeCount
15N chemical shifts148
1H chemical shifts836

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 206 residues - 16263.355 Da.

Residues 92-102 correspond to anN-terminal His-tag for purification purposes.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASNLYSPROSERLYSPROLYSTHR
4   ASNMETLYSHISVALALAGLYALAALAALA
5   ALAGLYALAVALVALGLYGLYLEUGLYGLY
6   TYRMETLEUGLYSERVALMETSERARGPRO
7   LEUILEHISPHEGLYASNASPTYRGLUASP
8   ARGTYRTYRARGGLUASNMETTYRARGTYR
9   PROASNGLNVALTYRTYRARGPROVALASP
10   GLNTYRSERASNGLNASNASNPHEVALHIS
11   ASPCYSVALASNILETHRVALLYSGLNHIS
12   THRVALTHRTHRTHRTHRLYSGLYGLUASN
13   PHETHRGLUTHRASPILELYSILEMETGLU
14   ARGVALVALGLUGLNMETCYSILETHRGLN
15   TYRGLNARGGLUSERGLNALATYRTYRGLN
16   ARGGLYALASERVALASNILETHRVALLYS
17   GLNHISTHRVALTHRTHRTHRTHRLYSGLY
18   GLUASNPHETHRGLUTHRASPILELYSILE
19   METGLUARGVALVALGLUGLNMETCYSILE
20   THRGLNTYRGLNARGGLUSERGLNALATYR
21   TYRGLNARGGLYALASER

Related Database Links:

GB CAA04276

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks