BMRB Entry 25685

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PDB ID: 2n4x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25685
MolProbity Validation Chart

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Title:
Structure of the Transmembrane Electron Transporter CcdA
Deposition date:
2015-07-01
Original release date:
2015-09-02
Authors:
Chou, James; Williamson, Jessica
Citation:

Citation: Williamson, Jessica; Cho, Seung-Hyung; Ye, Jiqing; Collet, Jean-Francois; Beckwith, Jonathan; Chou, James. "NMR Structure of the Transmembrane Electron Transporter CcdA"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
CcdA, polymer, 208 residues, 20955.492 Da.

Natural source:

Natural source:   Common Name: euryarchaeotes   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSC124

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CcdA: GSTSQEQPTAQLMAFALGIL SVFSPAVLPVVPLIFAGSRG RALDAFLIVAGLTISMLILG YTASLFFGFFRVVAMLFLLI FALILLSDELDEKVSIFASR MTSGLSWKIQTLPSFFFGML LAFLWLPAILPFAGIAISQT LLSENPLVMLSYGLGMAVTI AAVFKMGEKFVKANFQLIRK VTGAIVLLYLAYFALTEVLL LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts451
15N chemical shifts156
1H chemical shifts158

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 208 residues - 20955.492 Da.

Residues 1-10 represent a non-native sequence left over from protease cleavage that removed the N-terminal DsbD fusion protein. The C-terminal HHHHHH is a non-native affinity tag used for purification.

1   GLYSERTHRSERGLNGLUGLNPROTHRALA
2   GLNLEUMETALAPHEALALEUGLYILELEU
3   SERVALPHESERPROALAVALLEUPROVAL
4   VALPROLEUILEPHEALAGLYSERARGGLY
5   ARGALALEUASPALAPHELEUILEVALALA
6   GLYLEUTHRILESERMETLEUILELEUGLY
7   TYRTHRALASERLEUPHEPHEGLYPHEPHE
8   ARGVALVALALAMETLEUPHELEULEUILE
9   PHEALALEUILELEULEUSERASPGLULEU
10   ASPGLULYSVALSERILEPHEALASERARG
11   METTHRSERGLYLEUSERTRPLYSILEGLN
12   THRLEUPROSERPHEPHEPHEGLYMETLEU
13   LEUALAPHELEUTRPLEUPROALAILELEU
14   PROPHEALAGLYILEALAILESERGLNTHR
15   LEULEUSERGLUASNPROLEUVALMETLEU
16   SERTYRGLYLEUGLYMETALAVALTHRILE
17   ALAALAVALPHELYSMETGLYGLULYSPHE
18   VALLYSALAASNPHEGLNLEUILEARGLYS
19   VALTHRGLYALAILEVALLEULEUTYRLEU
20   ALATYRPHEALALEUTHRGLUVALLEULEU
21   LEUGLUHISHISHISHISHISHIS

Related Database Links:

GB AAB90190 AIG97935
REF WP_048064325

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