BMRB Entry 25657
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PDB ID: 2n3t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25657
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Chen, Xiang; Shi, Yanhong; Shi, Yuan; Elsasser, Suzanne; Vannoy, Jacob; Finley, Daniel; Tarasov, Sergey; Walters, Kylie. "Structures of Rpn1:ubiquitin and Rpn1:K48 diubiquitin define Rpn1 as a novel proteasome ubiquitin receptor." .
Assembly members:
protein, polymer, 131 residues, 13636.695 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pgex4t1
Entity Sequences (FASTA):
protein: DTKISSAAILGLGIAFAGSK
NDEVLGLLLPIAASTDLPIE
TAAMASLALAHVFVGTCNGD
ITTSIMDNFLERTAIELKTD
WVRFLALALGILYMGQGEQV
DDVLETISAIEHPMTSAIEV
LVGSCAYTGTG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 303 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 657 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 131 residues - 13636.695 Da.
| 1 | ASP | THR | LYS | ILE | SER | SER | ALA | ALA | ILE | LEU | ||||
| 2 | GLY | LEU | GLY | ILE | ALA | PHE | ALA | GLY | SER | LYS | ||||
| 3 | ASN | ASP | GLU | VAL | LEU | GLY | LEU | LEU | LEU | PRO | ||||
| 4 | ILE | ALA | ALA | SER | THR | ASP | LEU | PRO | ILE | GLU | ||||
| 5 | THR | ALA | ALA | MET | ALA | SER | LEU | ALA | LEU | ALA | ||||
| 6 | HIS | VAL | PHE | VAL | GLY | THR | CYS | ASN | GLY | ASP | ||||
| 7 | ILE | THR | THR | SER | ILE | MET | ASP | ASN | PHE | LEU | ||||
| 8 | GLU | ARG | THR | ALA | ILE | GLU | LEU | LYS | THR | ASP | ||||
| 9 | TRP | VAL | ARG | PHE | LEU | ALA | LEU | ALA | LEU | GLY | ||||
| 10 | ILE | LEU | TYR | MET | GLY | GLN | GLY | GLU | GLN | VAL | ||||
| 11 | ASP | ASP | VAL | LEU | GLU | THR | ILE | SER | ALA | ILE | ||||
| 12 | GLU | HIS | PRO | MET | THR | SER | ALA | ILE | GLU | VAL | ||||
| 13 | LEU | VAL | GLY | SER | CYS | ALA | TYR | THR | GLY | THR | ||||
| 14 | GLY |
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SPARKY: Backbone
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