BMRB Entry 25643

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PDB ID: 2n3e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25643
MolProbity Validation Chart

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Title:
Amino-terminal domain of Latrodectus hesperus MaSp1 with neutralized acidic cluster
Deposition date:
2015-05-29
Original release date:
2016-05-31
Authors:
Schaal, Daniel; Schwarzinger, Stephan; Bauer, Joschka; Roesch, Paul; Scheibel, Thomas; Schweimer, Kristian
Citation:

Citation: Schaal, Daniel; Schwarzinger, Stephan; Bauer, Joschka; Scheibel, Thomas; Schweimer, Kristian. "Solution structure of the amino-terminal domain of major spider silk amplulate with neutralized charge cluster"  .

Assembly members:

Assembly members:
entity, polymer, 137 residues, 14011.565 Da.

Natural source:

Natural source:   Common Name: western black widow spider   Taxonomy ID: 256737   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Latrodectus hesperus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GMGQANTPWSSKANADAFIN SFISAASNTGSFSQDQMENM SLIGNTLMAAMDNMGGRITP SKLQALDMAFASSVAQIAAS QGGDLGVTTNAIADALTSAF YQTTGVVNSRFISEIRSLIG MFAQASANDVYASAGSG

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts128
1H chemical shifts756

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 137 residues - 14011.565 Da.

No through-space distance information for unstructured termini

1   GLYMETGLYGLNALAASNTHRPROTRPSER
2   SERLYSALAASNALAASPALAPHEILEASN
3   SERPHEILESERALAALASERASNTHRGLY
4   SERPHESERGLNASPGLNMETGLUASNMET
5   SERLEUILEGLYASNTHRLEUMETALAALA
6   METASPASNMETGLYGLYARGILETHRPRO
7   SERLYSLEUGLNALALEUASPMETALAPHE
8   ALASERSERVALALAGLNILEALAALASER
9   GLNGLYGLYASPLEUGLYVALTHRTHRASN
10   ALAILEALAASPALALEUTHRSERALAPHE
11   TYRGLNTHRTHRGLYVALVALASNSERARG
12   PHEILESERGLUILEARGSERLEUILEGLY
13   METPHEALAGLNALASERALAASNASPVAL
14   TYRALASERALAGLYSERGLY

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