BMRB Entry 25629

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25629

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for G56C_T163C mutant of Adenylate Kinase at reduced condition
Deposition date:
2015-05-18
Original release date:
2017-05-19
Authors:
Kovermann, Michael; Wolf-Watz, Magnus
Citation:

Citation: Kovermann, Michael; Grundstrom, Christin; Sauer-Eriksson, A Elisabeth; Sauer, Uwe; Wolf-Watz, Magnus. "Structural basis for ligand binding to an enzyme by a conformational selection pathway"  Proc. Natl. Acad. Sci. U.S.A. 114, 6298-6303 (2017).
PubMed: 28559350

Assembly members:

Assembly members:
Adenylate_Kinase_G56C_T163C, polymer, 214 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEAK-91 vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Adenylate_Kinase_G56C_T163C: MRIILLGAPGAGKGTQAQFI MEKYGIPQISTGDMLRAAVK SGSELGKQAKDIMDACKLVT DELVIALVKERIAQEDCRNG FLLDGFPRTIPQADAMKEAG INVDYVLEFDVPDELIVDRI VGRRVHAPSGRVYHVKFNPP KVEGKDDVTGEELTTRKDDQ EECVRKRLVEYHQMTAPLIG YYSKEAEAGNTKYAKVDGTK PVAEVRADLEKILG

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts203
1H chemical shifts203

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Adenylate Kinase G56C_T163C1

Entities:

Entity 1, Adenylate Kinase G56C_T163C 214 residues - Formula weight is not available

1   METARGILEILELEULEUGLYALAPROGLY
2   ALAGLYLYSGLYTHRGLNALAGLNPHEILE
3   METGLULYSTYRGLYILEPROGLNILESER
4   THRGLYASPMETLEUARGALAALAVALLYS
5   SERGLYSERGLULEUGLYLYSGLNALALYS
6   ASPILEMETASPALACYSLYSLEUVALTHR
7   ASPGLULEUVALILEALALEUVALLYSGLU
8   ARGILEALAGLNGLUASPCYSARGASNGLY
9   PHELEULEUASPGLYPHEPROARGTHRILE
10   PROGLNALAASPALAMETLYSGLUALAGLY
11   ILEASNVALASPTYRVALLEUGLUPHEASP
12   VALPROASPGLULEUILEVALASPARGILE
13   VALGLYARGARGVALHISALAPROSERGLY
14   ARGVALTYRHISVALLYSPHEASNPROPRO
15   LYSVALGLUGLYLYSASPASPVALTHRGLY
16   GLUGLULEUTHRTHRARGLYSASPASPGLN
17   GLUGLUCYSVALARGLYSARGLEUVALGLU
18   TYRHISGLNMETTHRALAPROLEUILEGLY
19   TYRTYRSERLYSGLUALAGLUALAGLYASN
20   THRLYSTYRALALYSVALASPGLYTHRLYS
21   PROVALALAGLUVALARGALAASPLEUGLU
22   LYSILELEUGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks