BMRB Entry 25624

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25624

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Title:
Backbone 1H, 13C, and 15N chemical shift assignments for the double mutant G89A,D91R of KaiB from the Thermosynechococcus elongatus BP-1 cyanobacterial species
Deposition date:
2015-05-16
Original release date:
2015-07-14
Authors:
Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy
Citation:

Citation: Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy. "A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria"  Science 349, 324-328 (2015).
PubMed: 26113641

Assembly members:

Assembly members:
FTeKaiBGDF, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FTeKaiBGDF: DYKDDDDKMAPLRKTYVLKL YVAGNTPNSVRALKTLNNIL EKEFKGVYALKVIDVLKNPQ LAEEDKILATPTLAKVLPPP VRRIIGDLSNREKVLIALRL LYEEIGDQAEDDLGLEDYKD DDDK

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KaiB G89A D91R mutant, chain 11
2KaiB G89A D91R mutant, chain 21

Entities:

Entity 1, KaiB G89A D91R mutant, chain 1 124 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSMETALA
2   PROLEUARGLYSTHRTYRVALLEULYSLEU
3   TYRVALALAGLYASNTHRPROASNSERVAL
4   ARGALALEULYSTHRLEUASNASNILELEU
5   GLULYSGLUPHELYSGLYVALTYRALALEU
6   LYSVALILEASPVALLEULYSASNPROGLN
7   LEUALAGLUGLUASPLYSILELEUALATHR
8   PROTHRLEUALALYSVALLEUPROPROPRO
9   VALARGARGILEILEGLYASPLEUSERASN
10   ARGGLULYSVALLEUILEALALEUARGLEU
11   LEUTYRGLUGLUILEGLYASPGLNALAGLU
12   ASPASPLEUGLYLEUGLUASPTYRLYSASP
13   ASPASPASPLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks