BMRB Entry 25588

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PDB ID: 2n27
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25588
MolProbity Validation Chart

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Title:
Direct attack of vanilloids on the regulation of the pain receptor TRPV1
Deposition date:
2015-04-29
Original release date:
2016-06-30
Authors:
Hetenyi, Anasztazia; Nemeth, Lukacs; Weber, Edit; Szakonyi, Gerda; Winter, Zoltan; Josvay, Katalin; Olah, Zoltan; Martinek, Tamas
Citation:

Citation: Hetenyi, Anasztazia; Nemeth, Lukacs; Weber, Edit; Szakonyi, Gerda; Winter, Zoltan; Josvay, Katalin; Olah, Zoltan; Martinek, Tamas. "Competitive inhibition of TRPV1 - calmodulin interaction by vanilloids"  FEBS Lett. 590, 2768-2775 (2016).
PubMed: 27339229

Assembly members:

Assembly members:
Calmodulin, polymer, 148 residues, 16721.465 Da.
entity_CA, non-polymer, 40.078 Da.
entity_4DY, non-polymer, 305.412 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data sets:
Data typeCount
13C chemical shifts1205
15N chemical shifts421
1H chemical shifts1663

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2CALCIUM ION2
3CALCIUM ION2
4CALCIUM ION2
5CALCIUM ION2
6(6E)-N-(4-HYDROXY-3-METHOXYBENZYL)-8-METHYLNON-6-ENAMIDE3

Entities:

Entity 1, calmodulin 148 residues - 16721.465 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Entity 3, (6E)-N-(4-HYDROXY-3-METHOXYBENZYL)-8-METHYLNON-6-ENAMIDE - C18 H27 N O3 - 305.412 Da.

1   4DY

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SPARKY: Backbone or all simulated peaks