BMRB Entry 25587

Title:
Solution structures of Miz-1 zinc fingers 2 to 4
Deposition date:
2015-04-28
Original release date:
2016-03-22
Authors:
Bedard, Mikael; Lavigne, Pierre
Citation:

Citation: Bedard, Mikael; Lavigne, Pierre. "Solution structures of Miz-1 zinc fingers 2 to 4"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
Miz1-4, polymer, 112 residues, 12790.7447 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Data typeCount
13C chemical shifts395
15N chemical shifts99
1H chemical shifts524

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Miz1-41

Entities:

Entity 1, Miz1-4 112 residues - 12790.7447 Da.

1   METVALILEHISLYSCYSGLUASPCYSGLY
2   LYSGLUPHETHRHISTHRGLYASNPHELYS
3   ARGHISILEARGILEHISTHRGLYGLULYS
4   PROPHESERCYSARGGLUCYSSERLYSALA
5   PHESERASPPROALAALACYSLYSALAHIS
6   GLULYSTHRHISSERPROLEULYSPROTYR
7   GLYCYSGLUGLUCYSGLYLYSSERTYRARG
8   LEUILESERLEULEUASNLEUHISLYSLYS
9   ARGHISSERGLYGLUALAARGTYRARGCYS
10   GLUASPCYSGLYLYSLEUPHETHRTHRSER
11   GLYASNLEULYSARGHISGLNLEUVALHIS
12   SERGLY

Samples:

sample_1: Miz1-4, [U-13C; U-15N], 0.7 – 1.0 mM; acetic acid 10 mM; cacodylate 10 mM; KCl 50 mM; TCEP 2 mM; ZnCl2 5 equivalents; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
cbcaconh (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
hnha (H{[N]+[HA]})sample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
ccconh (HNCO)sample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - Structure calculation, refinement

CcpNmr_Analysis v2.1, CCPN - Chemical shift assigment, data analysis

DANGLE v1.1, CCPN - Data analysis

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Processing

ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - Structure calculation, refinement

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UniProt Q13105
AlphaFold Q9NUC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks