BMRB Entry 25559

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PDB ID: 2n1g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25559
MolProbity Validation Chart

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Title:
Structure of C-terminal domain of human polymerase Rev1 in complex with PolD3 RIR-motif
Deposition date:
2015-04-01
Original release date:
2016-04-12
Authors:
Pustovalova, Yulia; Korzhnev, Dmitry
Citation:

Citation: Pustovalova, Yulia; Magalhaes, Mariana; D'Souza, Sanjay; Rizzo, Alessandro; Korza, George; Walker, Graham; Korzhnev, Dmitry. "Interaction between the Rev1 C-terminal Domain and the PolD3 Subunit of Pol-zeta Suggests a Mechanism of Polymerase Exchange upon Rev1/Pol-zeta-Dependent Translesion Synthesis"  Biochemistry 55, 2043-2053 (2016).
PubMed: 26982350

Assembly members:

Assembly members:
Rev1-CT, polymer, 94 residues, 10954.707 Da.
PolD3, polymer, 16 residues, 1779.138 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rev1-CT: NLAGAVEFNDVKTLLREWIT TISDPMEEDILQVVKYCTDL IEEKDLEKLDLVIKYMKRLM QQSVESVWNMAFDFILDNVQ VVLQQTYGSTLKVT
PolD3: KGNMMSNFFGKAAMNK

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts103
1H chemical shifts731

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rev1-CT1
2PolD32

Entities:

Entity 1, Rev1-CT 94 residues - 10954.707 Da.

C-terminal domain of human polymerase Rev1 (residues 1158-1251)

1   ASNLEUALAGLYALAVALGLUPHEASNASP
2   VALLYSTHRLEULEUARGGLUTRPILETHR
3   THRILESERASPPROMETGLUGLUASPILE
4   LEUGLNVALVALLYSTYRCYSTHRASPLEU
5   ILEGLUGLULYSASPLEUGLULYSLEUASP
6   LEUVALILELYSTYRMETLYSARGLEUMET
7   GLNGLNSERVALGLUSERVALTRPASNMET
8   ALAPHEASPPHEILELEUASPASNVALGLN
9   VALVALLEUGLNGLNTHRTYRGLYSERTHR
10   LEULYSVALTHR

Entity 2, PolD3 16 residues - 1779.138 Da.

RIR-motif of accessory subunit PolD3 of human polymerase delta (residues 233-246)

1   LYSGLYASNMETMETSERASNPHEPHEGLY
2   LYSALAALAMETASNLYS

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