BMRB Entry 25533

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25533

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Title:
1H, 13C and 15N assignments of EGF domains 8 to 11 of human Notch-1
Deposition date:
2015-03-12
Original release date:
2015-06-04
Authors:
Handford, Penny; Redfield, Christina; Weisshuhn, Philip
Citation:

Citation: Weisshuhn, Philip; Handford, Penny; Redfield, Christina. "1H, 13C and 15N assignments of EGF domains 8-11 of human Notch-1"  Biomol. NMR assign. 9, 375-379 (2015).
PubMed: 25930016

Assembly members:

Assembly members:
hN-1_8-11, polymer, 159 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hN-1_8-11: SADVDECQLMPNACQNGGTC HNTHGGYNCVCVNGWTGEDC SENIDDCASAACFHGATCHD RVASFYCECPHGRTGLLCHL NDACISNPCNEGSNCDTNPV NGKAICTCPSGYTGPACSQD VDECSLGANPCEHAGKCINT LGSFECQCLQGYTGPRCEI

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts161
1H chemical shifts794

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hN-1 8-111
2Ca2+, 12
3Ca2+, 22
4Ca2+, 32

Entities:

Entity 1, hN-1 8-11 159 residues - Formula weight is not available

1   SERALAASPVALASPGLUCYSGLNLEUMET
2   PROASNALACYSGLNASNGLYGLYTHRCYS
3   HISASNTHRHISGLYGLYTYRASNCYSVAL
4   CYSVALASNGLYTRPTHRGLYGLUASPCYS
5   SERGLUASNILEASPASPCYSALASERALA
6   ALACYSPHEHISGLYALATHRCYSHISASP
7   ARGVALALASERPHETYRCYSGLUCYSPRO
8   HISGLYARGTHRGLYLEULEUCYSHISLEU
9   ASNASPALACYSILESERASNPROCYSASN
10   GLUGLYSERASNCYSASPTHRASNPROVAL
11   ASNGLYLYSALAILECYSTHRCYSPROSER
12   GLYTYRTHRGLYPROALACYSSERGLNASP
13   VALASPGLUCYSSERLEUGLYALAASNPRO
14   CYSGLUHISALAGLYLYSCYSILEASNTHR
15   LEUGLYSERPHEGLUCYSGLNCYSLEUGLN
16   GLYTYRTHRGLYPROARGCYSGLUILE

Entity 2, Ca2+, 1 - Ca - 40.078 Da.

1   CA

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