BMRB Entry 25490

Title:
Structure of Conantokin Rl-B
Deposition date:
2015-02-14
Original release date:
2015-06-15
Authors:
Castellino, Francis; Yuan, Yue; Kunda, Shailaja; Zajicek, Jaroslav
Citation:

Citation: Castellino, Francis; Yuan, Yue; Kunda, Shailaja; Zajicek, Jaroslav. "Hydroxyproline-induced Helical Disruption in Conantokin Rl-B Affects Subunit-selective Antagonistic Activities Toward Ion Channels of NMDA Receptors"  J. Biol. Chem. 290, 18156-18172 (2015).
PubMed: 26048991

Assembly members:

Assembly members:
Conantokin_Rl-B, polymer, 19 residues, 2184.304 Da.

Natural source:

Natural source:   Common Name: gastropods   Taxonomy ID: 745791   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus rolani

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Conantokin_Rl-B: GEXXLAXKAXXFARXLANX

Data sets:
Data typeCount
13C chemical shifts74
15N chemical shifts18
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Conantokin Rl-B1

Entities:

Entity 1, Conantokin Rl-B 19 residues - 2184.304 Da.

1   GLYGLUCGUCGULEUALACGULYSALAHYP
2   CGUPHEALAARGCGULEUALAASNNH2

Samples:

sample_1: entity 2 mM; HEPES, [U-100% 2H], 10 mM; DSS 0.01 mg/mL; sodium chloride 10 mM; magnesium chloride 40 mM; D2O, [U-100% 2H], 5%; H2O, [U-100% 2H], 95%

sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - peak picking

TOPSPIN, Bruker Biospin - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks