BMRB Entry 25485
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PDB ID: 5ue2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25485
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Prior, Stephen; Fulcher, Yan; Koppisetti, Rama; Jurkevich, A.; Van Doren, Steven. "Charge-Triggered Membrane Insertion of Matrix Metalloproteinase-7, Supporter of Innate Immunity and Tumors" Structure 23, 2099-2110 (2015).
PubMed: 26439767
Assembly members:
proMMP-7(E195A), polymer, 247 residues, 27589.332 Da.
CALCIUM ION, non-polymer, 40.078 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-a
Entity Sequences (FASTA):
proMMP-7(E195A): PQEAGGMSELQWEQAQDYLK
RFYLYDSETKNANSLEAKLK
EMQKFFGLPITGMLNSRVIE
IMQKPRCGVPDVAEYSLFPN
SPKWTSKVVTYRIVSYTRDL
PHITVDRLVSKALNMWGKEI
PLHFRKVVWGTADIMIGFAR
GAHGDSYPFDGPGNTLAHAF
APGTGLGGDAHFDEDERWTD
GSSLGINFLYAATHALGHSL
GMGHSSDPNAVMYPTYGNGD
PQNFKLSQDDIKGIQKLYGK
RSNSRKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1003 |
| 15N chemical shifts | 237 |
| 1H chemical shifts | 1506 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | CALCIUM ION_1 | 2 |
| 3 | CALCIUM ION_2 | 2 |
| 4 | ZINC ION_1 | 3 |
| 5 | ZINC ION_2 | 3 |
Entities:
Entity 1, entity_1 247 residues - 27589.332 Da.
| 1 | PRO | GLN | GLU | ALA | GLY | GLY | MET | SER | GLU | LEU | ||||
| 2 | GLN | TRP | GLU | GLN | ALA | GLN | ASP | TYR | LEU | LYS | ||||
| 3 | ARG | PHE | TYR | LEU | TYR | ASP | SER | GLU | THR | LYS | ||||
| 4 | ASN | ALA | ASN | SER | LEU | GLU | ALA | LYS | LEU | LYS | ||||
| 5 | GLU | MET | GLN | LYS | PHE | PHE | GLY | LEU | PRO | ILE | ||||
| 6 | THR | GLY | MET | LEU | ASN | SER | ARG | VAL | ILE | GLU | ||||
| 7 | ILE | MET | GLN | LYS | PRO | ARG | CYS | GLY | VAL | PRO | ||||
| 8 | ASP | VAL | ALA | GLU | TYR | SER | LEU | PHE | PRO | ASN | ||||
| 9 | SER | PRO | LYS | TRP | THR | SER | LYS | VAL | VAL | THR | ||||
| 10 | TYR | ARG | ILE | VAL | SER | TYR | THR | ARG | ASP | LEU | ||||
| 11 | PRO | HIS | ILE | THR | VAL | ASP | ARG | LEU | VAL | SER | ||||
| 12 | LYS | ALA | LEU | ASN | MET | TRP | GLY | LYS | GLU | ILE | ||||
| 13 | PRO | LEU | HIS | PHE | ARG | LYS | VAL | VAL | TRP | GLY | ||||
| 14 | THR | ALA | ASP | ILE | MET | ILE | GLY | PHE | ALA | ARG | ||||
| 15 | GLY | ALA | HIS | GLY | ASP | SER | TYR | PRO | PHE | ASP | ||||
| 16 | GLY | PRO | GLY | ASN | THR | LEU | ALA | HIS | ALA | PHE | ||||
| 17 | ALA | PRO | GLY | THR | GLY | LEU | GLY | GLY | ASP | ALA | ||||
| 18 | HIS | PHE | ASP | GLU | ASP | GLU | ARG | TRP | THR | ASP | ||||
| 19 | GLY | SER | SER | LEU | GLY | ILE | ASN | PHE | LEU | TYR | ||||
| 20 | ALA | ALA | THR | HIS | ALA | LEU | GLY | HIS | SER | LEU | ||||
| 21 | GLY | MET | GLY | HIS | SER | SER | ASP | PRO | ASN | ALA | ||||
| 22 | VAL | MET | TYR | PRO | THR | TYR | GLY | ASN | GLY | ASP | ||||
| 23 | PRO | GLN | ASN | PHE | LYS | LEU | SER | GLN | ASP | ASP | ||||
| 24 | ILE | LYS | GLY | ILE | GLN | LYS | LEU | TYR | GLY | LYS | ||||
| 25 | ARG | SER | ASN | SER | ARG | LYS | LYS |
Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.
| 1 | CA |
Entity 3, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| NCBI | NP_002414.1 |
| BMRB | 25488 25489 |
| PDB | 1MMP 1MMQ 1MMR 2DDY 2MZE 2Y6C 2Y6D |
| DBJ | BAD96700 BAG56875 BAI46673 |
| EMBL | CAA30678 CAA77942 |
| GB | AAC37543 AAH03635 AAV40839 AAX36381 AAX36443 |
| REF | NP_002414 XP_002822443 XP_003828431 XP_004052078 XP_508721 |
| SP | P09237 |
| AlphaFold | P09237 P09237 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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or all simulated peaks