BMRB Entry 25482

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PDB ID: 2mzb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25482
MolProbity Validation Chart

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Title:
Solution structural studies of GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) from Methanocaldococcus jannaschii
Deposition date:
2015-02-11
Original release date:
2015-11-09
Authors:
Singarapu, Kiran Kumar; Otte, Michele; Tonelli, Marco; Westler, William; Escalante-Semerena, Jorge; Markley, John
Citation:

Citation: Singarapu, Kiran Kumar; Otte, Michele; Tonelli, Marco; Westler, William; Escalante-Semerena, Jorge; Markley, John. "Solution Structural Studies of GTP:Adenosylcobinamide-Phosphateguanylyl Transferase (CobY) from Methanocaldococcus jannaschii"  Plos One 10, e0141297-e0141297 (2015).
PubMed: 26513744

Assembly members:

Assembly members:
CobY, polymer, 196 residues, 21770.621 Da.

Natural source:

Natural source:   Common Name: euryarchaeotes   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCobY14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CobY: MDALIMAGGKGTRMGGVEKP LIKLCGRCLIDYVVSPLLKS KVNNIFIATSPNTPKTKEYI NSAYKDYKNIVVIDTSGKGY IEDLNECIGYFSEPFLVVSS DLINLKSKIINSIVDYFYCI KAKTPDVEALAVMIPKEKYP NPSIDFNGLVPAGINVVSPK HGYQKEEIMVIDELIFNINT KDDLKLAEMLLKKDGL

Data sets:
Data typeCount
13C chemical shifts705
15N chemical shifts156
1H chemical shifts1364

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CobY1

Entities:

Entity 1, CobY 196 residues - 21770.621 Da.

1   METASPALALEUILEMETALAGLYGLYLYS
2   GLYTHRARGMETGLYGLYVALGLULYSPRO
3   LEUILELYSLEUCYSGLYARGCYSLEUILE
4   ASPTYRVALVALSERPROLEULEULYSSER
5   LYSVALASNASNILEPHEILEALATHRSER
6   PROASNTHRPROLYSTHRLYSGLUTYRILE
7   ASNSERALATYRLYSASPTYRLYSASNILE
8   VALVALILEASPTHRSERGLYLYSGLYTYR
9   ILEGLUASPLEUASNGLUCYSILEGLYTYR
10   PHESERGLUPROPHELEUVALVALSERSER
11   ASPLEUILEASNLEULYSSERLYSILEILE
12   ASNSERILEVALASPTYRPHETYRCYSILE
13   LYSALALYSTHRPROASPVALGLUALALEU
14   ALAVALMETILEPROLYSGLULYSTYRPRO
15   ASNPROSERILEASPPHEASNGLYLEUVAL
16   PROALAGLYILEASNVALVALSERPROLYS
17   HISGLYTYRGLNLYSGLUGLUILEMETVAL
18   ILEASPGLULEUILEPHEASNILEASNTHR
19   LYSASPASPLEULYSLEUALAGLUMETLEU
20   LEULYSLYSASPGLYLEU

Related Database Links:

PDB 2MZB 3RSB
GB AAB99118
SP Q58517
AlphaFold Q58517

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks