BMRB Entry 25469

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PDB ID: 2mz1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25469
MolProbity Validation Chart

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Title:
Solution structure of hnRNP C RRM in complex with 5'-UUUUC-3' RNA
Deposition date:
2015-02-05
Original release date:
2015-04-09
Authors:
Cienikova, Zuzana; Damberger, Fred; Hall, Jonathan; Allain, Frederic; Maris, Christophe
Citation:

Citation: Cienikova, Zuzana; Damberger, Fred; Hall, Jonathan; Allain, Frederic; Maris, Christophe. "Structural and mechanistic insights into poly(uridine) tract recognition by the hnRNP C RNA recognition motif."  J. Am. Chem. Soc. 136, 14536-14544 (2014).
PubMed: 25216038

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, 11292.014 Da.
RNA_(5'-R(*UP*UP*UP*UP*C)-3'), polymer, 5 residues, 1484.899 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ASNVTNKTDPRSMNSRVFIG NLNTLVVKKSDVEAIFSKYG KIVGCSVHKGFAFVQYVNER NARAAVAGEDGRMIAGQVLD INLAAEPKVNRGKAGVKRSA AEMYG
RNA_(5'-R(*UP*UP*UP*UP*C)-3'): UUUUC

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts117
1H chemical shifts776

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNP C RRM1
2RNA (5'-R(*UP*UP*UP*UP*C)-3')2

Entities:

Entity 1, hnRNP C RRM 105 residues - 11292.014 Da.

1   ALASERASNVALTHRASNLYSTHRASPPRO
2   ARGSERMETASNSERARGVALPHEILEGLY
3   ASNLEUASNTHRLEUVALVALLYSLYSSER
4   ASPVALGLUALAILEPHESERLYSTYRGLY
5   LYSILEVALGLYCYSSERVALHISLYSGLY
6   PHEALAPHEVALGLNTYRVALASNGLUARG
7   ASNALAARGALAALAVALALAGLYGLUASP
8   GLYARGMETILEALAGLYGLNVALLEUASP
9   ILEASNLEUALAALAGLUPROLYSVALASN
10   ARGGLYLYSALAGLYVALLYSARGSERALA
11   ALAGLUMETTYRGLY

Entity 2, RNA (5'-R(*UP*UP*UP*UP*C)-3') 5 residues - 1484.899 Da.

1   UUUUC

Related Database Links:

UNP P07910
BMRB 25436
PDB 2MXY 2MZ1
DBJ BAB27553 BAB28370 BAB31934 BAC40233 BAC40499
EMBL CAD61934 CAD62326 CAH91328
GB AAA36576 AAA52680 AAC61695 AAD03717 AAH03394
REF NP_001029567 NP_001070910 NP_001070911 NP_001075507 NP_001125784
SP G3V9R8 O77768 P07910 Q5RA82 Q9Z204
TPG DAA25480
AlphaFold Q9BTS3 G3V9R8 O77768 P07910 Q5RA82 Q9Z204

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