BMRB Entry 25430
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25430
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Citation: Choy, Meng Shyan; Yusoff, Permeen; Lee, Irene C.; Newton, Jocelyn C.; Goh, Catherine W.; Page, Rebecca; Shenolikar, Shirish; Peti, Wolfgang. "Structural and Functional Analysis of the GADD34:PP1 eIF2a Phosphatase" Cell Reports 11, 1885-1891 (2015).
Assembly members:
GADD34, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress411
Entity Sequences (FASTA):
GADD34: MKARKVRFSEKVTVHFLAVW
AGPAQAARQGPWEQLARDRS
RFARRITQAQEELSPCLTPA
ARARAWARLRNHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 203 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 252 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GADD34 | 1 |
Entities:
Entity 1, GADD34 77 residues - Formula weight is not available
Residue 1 represent a non native Met from the start codon. Residue 72-77 represent non-native affinity (His) tag.
| 1 | MET | LYS | ALA | ARG | LYS | VAL | ARG | PHE | SER | GLU | ||||
| 2 | LYS | VAL | THR | VAL | HIS | PHE | LEU | ALA | VAL | TRP | ||||
| 3 | ALA | GLY | PRO | ALA | GLN | ALA | ALA | ARG | GLN | GLY | ||||
| 4 | PRO | TRP | GLU | GLN | LEU | ALA | ARG | ASP | ARG | SER | ||||
| 5 | ARG | PHE | ALA | ARG | ARG | ILE | THR | GLN | ALA | GLN | ||||
| 6 | GLU | GLU | LEU | SER | PRO | CYS | LEU | THR | PRO | ALA | ||||
| 7 | ALA | ARG | ALA | ARG | ALA | TRP | ALA | ARG | LEU | ARG | ||||
| 8 | ASN | HIS | HIS | HIS | HIS | HIS | HIS |
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