BMRB Entry 25421

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PDB ID: 2mxo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25421
MolProbity Validation Chart

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Title:
NMR structure of spider toxin- G7W/N24S mutant of TRTX-Hhn2b
Deposition date:
2015-01-08
Original release date:
2015-12-21
Authors:
Klint, Julie; Chin, Yanni; Mobli, Medhi
Citation:

Citation: Klint, Julie; Chin, Yanni; Mobli, Medhi. "Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7"  Mol. Pharmacol. 88, 1002-1010 (2015).
PubMed: 26429937

Assembly members:

Assembly members:
GWHhn2b, polymer, 34 residues, 3793.473 Da.

Natural source:

Natural source:   Common Name: Chinese bird spider   Taxonomy ID: 209901   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Haplopelma hainanum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pR7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GWHhn2b: AECKGFWKSCVPGKNECCSG YACSSRDKWCKVLL

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts35
1H chemical shifts222

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1G7W/N24S Hhn2b1

Entities:

Entity 1, G7W/N24S Hhn2b 34 residues - 3793.473 Da.

1   ALAGLUCYSLYSGLYPHETRPLYSSERCYS
2   VALPROGLYLYSASNGLUCYSCYSSERGLY
3   TYRALACYSSERSERARGASPLYSTRPCYS
4   LYSVALLEULEU

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