BMRB Entry 25419

Title:
NMR solution structure of -TRTX-Tp1a from the tarantula Thrixopelma pruriens
Deposition date:
2015-01-08
Original release date:
2015-06-01
Authors:
Rosengren, K. Johan
Citation:

Citation: Cardoso, Fernanda; Dekan, Zoltan; Rosengren, K. Johan; Erickson, Andelain; Vetter, Irina; Deuis, Jennifer; Herzig, Volker; Alewood, Paul; King, Glenn; Lewis, Richard. "Identification and Characterization of ProTx-III [ mu-TRTX-Tp1a], A New Voltage-Gated Sodium Channel Inhibitor from Venom of the Tarantula Thrixopelma Pruriens"  Mol. Pharmacol. 88, 291-303 (2015).
PubMed: 25979003

Assembly members:

Assembly members:
entity, polymer, 33 residues, 3817.504 Da.

Natural source:

Natural source:   Common Name: green velvet   Taxonomy ID: 213387   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Thrixopelma pruriens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: DCLKFGWKCNPRNDKCCSGL KCGSNHNWCKLHL

Data sets:
Data typeCount
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 33 residues - 3817.504 Da.

1   ASPCYSLEULYSPHEGLYTRPLYSCYSASN
2   PROARGASNASPLYSCYSCYSSERGLYLEU
3   LYSCYSGLYSERASNHISASNTRPCYSLYS
4   LEUHISLEU

Samples:

sample_1: entity 0.4 mg/mL

sample_conditions_1: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz