BMRB Entry 25417

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25417

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Title:
NMR resonance assignments of CadC 1-159 from E.coli
Deposition date:
2015-01-07
Original release date:
2015-05-18
Authors:
Schlundt, Andreas; Sattler, Michael; Heydenreich, Thomas
Citation:

Citation: Schlundt, Andreas; Heydenreich, Thomas; Buchner, Sophie; Janowski, Robert; Niessing, Dierk; Sattler, Michael; Jung, Kirsten. "Structural and functional analysis of the signal transducing linker in the pH-responsive one component system CadC of Escherichia coli"  J. Mol. Biol. 427, 2548-2561 (2015).
PubMed: 25979249

Assembly members:

Assembly members:
CadC, polymer, 162 residues, 18160.6 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETTrx1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CadC: GAMAQQPVVRVGEWLVTPSI NQISRNGRQLTLEPRLIDLL VFFAQHSGEVLSRDELIDNV WKRSIVTNHVVTQSISELRK SLKDNDEDSPVYIATVPKRG YKLMVPVIWYSEEEGEEIML SSPPPIPEAVPATDSPSHSL NIQNTATPPEQSPVKSKRFT TF

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts156
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CadC1-1591

Entities:

Entity 1, CadC1-159 162 residues - 18160.6 Da.

Sequence starts with -2. GAM is cloning remnant, an additional A is part of the restriction site. The original sequence number starts with Q2.

1   GLYALAMETALAGLNGLNPROVALVALARG
2   VALGLYGLUTRPLEUVALTHRPROSERILE
3   ASNGLNILESERARGASNGLYARGGLNLEU
4   THRLEUGLUPROARGLEUILEASPLEULEU
5   VALPHEPHEALAGLNHISSERGLYGLUVAL
6   LEUSERARGASPGLULEUILEASPASNVAL
7   TRPLYSARGSERILEVALTHRASNHISVAL
8   VALTHRGLNSERILESERGLULEUARGLYS
9   SERLEULYSASPASNASPGLUASPSERPRO
10   VALTYRILEALATHRVALPROLYSARGGLY
11   TYRLYSLEUMETVALPROVALILETRPTYR
12   SERGLUGLUGLUGLYGLUGLUILEMETLEU
13   SERSERPROPROPROILEPROGLUALAVAL
14   PROALATHRASPSERPROSERHISSERLEU
15   ASNILEGLNASNTHRALATHRPROPROGLU
16   GLNSERPROVALLYSSERLYSARGPHETHR
17   THRPHE

Related Database Links:

UNP P23890
AlphaFold Q2M6H0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks