BMRB Entry 25396

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25396
MolProbity Validation Chart

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Title:
assignment of the transmembrane domain of the mouse erythropoietin receptor
Deposition date:
2014-12-17
Original release date:
2015-09-21
Authors:
Li, Qingxin; Wong, Yinglei; Lee, Michelle; Kang, Congbao
Citation:

Citation: Li, Qingxin; Wong, Yinglei; Lee, Michelle; Li, Michelle; kang, congbao. "Solution structure of the transmembrane domain of the mouse erythropoietin receptor in detergent micelles"  Sci. Rep. 5, 13586-13586 (2015).
PubMed: 26316120

Assembly members:

Assembly members:
mouse_EpoR, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mouse_EpoR: MSEPASLLTASDLDPLILTL SLILVLISLLLTVLALLSHR RTLQQKIWPHHHHHH

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts45
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse EpoR1

Entities:

Entity 1, mouse EpoR 55 residues - Formula weight is not available

1   METSERGLUPROALASERLEULEUTHRALA
2   SERASPLEUASPPROLEUILELEUTHRLEU
3   SERLEUILELEUVALLEUILESERLEULEU
4   LEUTHRVALLEUALALEULEUSERHISARG
5   ARGTHRLEUGLNGLNLYSILETRPPROHIS
6   HISHISHISHISHIS

Samples:

15N_labeled_sample: mouseEpoR, [U-100% 15N], 0.5 mM; sodium phosphate 20 mM; DPC 150 mM; H2O 90%; D2O, U-2H, 10%

13C-15N: mouseEpoR, [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 20 mM; DPC, [U-100% 2H], 240 mM; H2O 90%; D2O, U-2H, 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_labeled_sampleisotropicsample_conditions_1
2D 1H-15N HSQC15N_labeled_sampleisotropicsample_conditions_1
3D HNCACB13C-15Nisotropicsample_conditions_1
3D HNCA13C-15Nisotropicsample_conditions_1
3D HNCO13C-15Nisotropicsample_conditions_1
3D HBHA(CO)NH13C-15Nisotropicsample_conditions_1
3D 1H-15N NOESY13C-15Nisotropicsample_conditions_1
3D HN(CO)CA13C-15Nisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAE23871
EMBL CAA37248
GB AAA37571 AAB20029 AAH03953 AAH46282 EDL25236
REF NP_034279 XP_006510061
SP P14753
AlphaFold P14753

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks